低氘白酒对人肺腺癌细胞的抑制作用

Inhibitory Effect of Deuterium-depleted Alcohol on A549 and H460 Cells

目的:探讨低氘白酒在体内外对肺腺癌的抑制作用。方法:在体外,采用MTT法检测白酒、低氘水(DDW)和低氘白酒(DDA)抑制人肺癌A549细胞的增殖情况;采用TUNEL法和流式细胞仪分别检测白酒、DDW和DDA诱导人肺癌A549细胞的凋亡率。在体内,建立肺癌移植瘤模型,造模当日给药。9d后,开始测量瘤体生长曲线。21d后,断颈处死小鼠,称体质量及瘤质量,固定小鼠瘤组织及肝肺组织,HE染色检测病理变化。结果:在体外,72h、180mmol/L DDA能明显抑制A549增殖,细胞增殖率为(70.58±2.73)%,与空白组及白酒组相比较,具有显著性差异(P<0.05);TUNEL法检测A549细胞凋亡率,表明DDA能促进细胞凋亡,与空白组、DDW组、白酒组相比较,具有显著性差异(P<0.05);流式细胞仪检测到DDA能促进细胞凋亡。在体内,DDA组与DDW组肿瘤抑制率分别为31.014%和27.639%,与空白组相比较,具有显著性差异(P<0.05)。HE染色结果显示,DDW组与DDA低剂量组,肿瘤细胞排列松散,周围有坏死组织。同时,DDW及DDA对裸鼠的肝肺组织无影响。结论:低剂量DDA在体外能抑制肺腺癌A549细胞增殖,在体内能抑制人肺腺癌H460裸鼠皮下移植瘤生长,并诱导癌细胞凋亡。

Objective： To observe the inhibitory effect of deuterium-depleted alcohol on the proliferation of lung cancer cells, A549 and H460. Methods： in vitro, the inhibitory effects of alcohol, deuterium-depleted water （DDW） and deuterium- depleted alcohol （DDA） on the proliferation ofA549 cells were measured by MTT assay, and the apoptosis was determined by TUNEL method and flow cytometry （FCM）. in vivo, human lung cancer H460 ceils were implanted into nude mice. After 9 days, a growth curve was established to reveal the growth characteristics of xenograft. After 21 days, the mice were sacrificed, and the tumors were weighed and calculated for tumor-suppression rate. Pathological changes of tumor tissues were observed by means of HE staining. Pathological changes of liver and lung tissues were also observed. Results： The proliferation ofA549 cells was remarkably inhibited by DDA at the concentration of 180 mmol/L, when compared with the control group and the alcohol group （P 〈 0.05）. A549 cells induced by DDA revealed an obvious apoptosis, when compared with the control group, the alcohol group and DDW group （P 〈 0.05）. Moreover, FCM assay results showed that DDA could promote apoptosis. The in vivo data obtaiend showed higher tumor inhibition rates of 31.014% and 27.639%, respectively in DDA and DDW group than in the control group （P 〈 0.05）. HE staining showed obvious necrosis and loose arrangement of tumor cells in DDA and DDW groups, but no obvious changes in lung or liver tissues were observed. Conclusion： DDA has an obvious inhibitory effect on the proliferation of lung cancer cells.