左卡尼汀对大鼠单侧输尿管梗阻肾间质纤维化的影响

Function of L-carnitine on rat renal interstitial fibrosis with unilateral ureteral obstruction

目的观察左卡尼汀（Lc）对单侧输尿管梗阻（UUO）大鼠肾间质纤维化的影响，探讨治疗UUO时配对盒基因-2（PAX2）、仪．平滑肌肌动蛋白（01．SMA）、转化生长因子-β1（TGF-β1）的表达及意义。方法将36只SD大鼠分为3组：假手术组（Sham）、UUO组（uuo）、左卡尼汀组（LC）。各组分别在第7、14天后取肾组织检测a-SMA、PAX2、TGF-β1表达。结果LC组中鼠肾组织PAX2mRNA水平较UUO组明显降低（P〈0．05），但比Sham组高（P〈0．05）。免疫组织化学结果示各组大鼠肾组织PAX2蛋白水平差异有统计学意义（P〈0．05），第7天平均吸光度（A）值分别为UUO组0．5547±0．0593、LC组0．4225±0．0298、Sham组0．2556-4-0．0359。第14天平均4值分别为：UUO组0．6376±0．0448、LC组0．4731±O．0342、Sham组0．2480±0．0256。第7、14天鼠肾组织中仅-SMA和TGF-β1蛋白表达水平均提示：Lc组较UUO组明显降低（P〈0．05），但比Sham组高（P〈0．05）。结论LC对肾小管纤维化及肾损伤有明显的保护作用，同时可抑制PAX2的再表达，并降低d—SMA、TGF-β1表达水平，可降低肾小管纤维化程度，减少肾小管细胞凋亡。

Objective This study was performed to investigate the influence on rat renal interstitial fibrosis with unilateral ureteral obstruction by L-carnitine （ LC ） , and examine significance and changes of paired box gene-2 （PAX2） , a-smooth muscle actin （ct-SMA） and transforming growth factor--β1 （TGF--β1） in this process. Methods Thirty-six rats were divided into three groups randomly： Sham operation group （Sham） , model group subjected to unilateral ureteral obstruction （UUO） , and L-carnitine-treated group （LC）. Kidney tissues of every group were taken by operation in 7th and 14th day, and protein expression levels of a-SMA, PAX2 and TGF-β1 were detected by different ways. Results PAX2 mRNA was signifi- cantly reduced in rat kidney tissue in LC group compared to UUO group （ P 〈 0. 05 ）, but increased compared to Sham group （ P 〈 0. 05 ）. Immunochemistry shows significant discrepancy in PAX2 between each groups （P 〈0. 05）. Average absorbance was （0. 5547±0. 0593）, （0. 4225 ±0. 0298） and （0. 2556±0. 0359） in UUO, LC and Sham group respectively at 7 days as well as （0. 6376±0. 0448）, （0. 4731±0. 0342） and （0. 2480±0. 0256） correspondingly at 14 days. Protein expression of a-SMA and TGF-β1 in rat kidney tissue indicates was significantly reduced in LC group compared to UUO group （ P 〈 0. 05 ） , but increased compared to Sham group （ P 〈 0. 05 ） at days 7 and 14. Conclusion L-carnitine could effectively decrease renal injury, down-regulate the level of re-expression of PAX2, inhibit expression of a-SMA, TGF-β1. Therefore it could reduce renal interstitial fibrosis and cell apoptosis by some mechanism.