水通道蛋白1在大鼠慢传输便秘模型结肠黏膜中的表达

Expression of aquaporin 1 in colonic mucosa of rats with slow transit constipation

目的建立大鼠慢传输型便秘（STC）模型，观察STC组大鼠结肠黏膜上的水通道蛋白1（AQP1）表达变化，探讨其与STC的关系。方法将32只sD大鼠随机分为对照组（n=16）和STC组（n=16），采用复方地芬诺酯灌胃的方法建立大鼠慢传输便秘模型成功后，利用逆转录聚合酶链反应（RT—PCR）方法测定STC组及对照组大鼠升降结肠黏膜的AQP1mRNA表达。结果STC组大鼠日均粪便质量[（1_279±0．745）g/100g]比对照组[（1．571±0．783）g/100g]明显减少（P〈0．05）；大鼠消化道传输速度检测STC组首粒黑便排出时间为（461．6±150．8）min，较对照组大鼠[（351．3±119．9）min]显著延长（P〈0．05）。实验组和对照组升结肠AQP1灰度比平均值分别是0．281±0．212和0．502±0．239（P〈0．05）、降结肠AQP1灰度比平均值分别是0．429±0．309和0．509±0．308（P〉0．05）。结论AQP1在STC组升结肠黏膜表达下调，可能对STC的发生、发展有一定的作用。

Objective To investigate the expression of aquaporinl in colonic mucosa of rats with slow transit constipation （ STC）, and its role in the pathologenesis of STC. Methods Thirty-two Spragne- Dawley rats were randomly divided into two groups： control group （n = 16） and STC group （n = 16）. STC model was created by giving the rats the solution of compound diphenoxylate. The reverse transcriptase-pol- ymerase chain reaction （RT-PCR） was used to detect the expression of apuaporin 1 （AQP1） mRNA in co-lonic mucosa of rats with STC （trial group） and the normal rats （ control group）. Results The fecal mass of STC rats was （1. 279±0. 743 ） g/100 g, significantly less than that in the normal rats [ （1. 571 ±0.783） g/100 g] （P〈0.05）. In STC rats, the time of discharge of the first black fecal was 461.6 ±150. 8 min, significantly longer than that in the normal rats [ （351.3±119. 9） mini （P 〈 0. 05 ）. RT- PCR revealed that the average values of gray scale ratios of AQP1 in proximal colon of trial group and control group were 0.281±0. 212 and 0. 502 ±0. 239 respectively （ P 〈 0.05 ）. The average values of gray scale ratios of AQP1 in distal colon of trial group and control group were 0. 429 ± 0. 309 and 0. 509 ±0. 308 respectively （P 〉 0. 05）. Conclusion Expression of AQP1 in proximal colon of rats with STC was down- regulated, which may play roles in water absorption.