吡格列酮在大鼠心脏移植排斥反应中的作用

Effect of pioglitazone on rats with heart transplantation rejection

目的观察吡格列酮（Pio）在心脏移植排斥反应中的作用，探讨Pio减轻心脏移植排斥反应的机制。方法建立大鼠腹部心脏移植模型，分组给予全量环孢素A（CsA）5mg／kg、半量CsA、Pio，以及Pio与不同剂量的CsA联合应用，术后3、7、14、28d观察移植心脏存活时间、病理变化、脾脏CD25＋、CD28＋T细胞的表达以及血清白细胞介素（IL）-2的变化。结果Pio与CsA联合应用能够明显延长移植心脏的存活时间，减轻心脏的排斥反应，降低脾脏CD25＋、CD28＋的表达，降低血清IL-2的浓度（P〈0．05）。结论Pio与CsA联合应用可减少CsA的用量，减轻排斥反应，改善受体的生存状态。

Objective To study the role of pioglitazone （Pio） in heart transplantation rejection and the mechanism. Methods In the rat heart transplantation model, pio was combined with cyclosporin A （CsA） in different doses as the experimental group, and CsA was used alone in different doses as the con- trol group. On the post-transplantation day 3, 7, 14 and 28, the survival, patholgogical changes, expres- sion of splenic CD25 ＋ and CD28 ＋ T ceils and serum interleukin （IL）-2 were observed. Results The combined use of Pio and CsA could significantly prologn the survival of the donor hearts, alleviated rejec- tion of the transplanted hearts, reduced the expression of splenic CD25 ＋ and CD28 ＋ T ceils, and de- creased the levels of serum IL-2 （P 〈 0. 05）. Conclusion The combined use of Pio and CsA could reduce the usage of CsA, alleviate rejection and impromve the survival state of the recipients.