大鼠高肺血流量肺动脉高压模型的建立

Establishment of high flow pulmonary hypertension model in rats

目的建立大鼠高肺血流量肺动脉高压模型。方法采取左颈总动脉-颈外静脉分流、左肺动脉结扎以及左颈总动脉-颈外静脉分流联合左肺动脉结扎3种手术方式建立大鼠高肺血流量模型，观察术后8周肺动脉平均压（mPAP）、右心室肥厚指数（RV／LV＋S）、肺血管管壁厚度占血管外径的百分比（WT％）和管壁面积占血管总面积的百分比（WA％）；以及左颈总动静脉分流联合左肺动脉结扎术后4、8、12周上述指标的变化。结果与对照组比较，左颈总动脉-颈外静脉分流联合左肺动脉结扎在术后8、12周产生明显肺动脉高压，mPAP及RV／LV＋S逐渐增加，且差异有统计学意义（P〈0．01），WT％和WA％差异也均有统计学意义（P〈0．01）。结论左颈总动脉．颈外静脉分流联合左肺动脉结扎可建立高肺血流量肺动脉高压模型，且操作简单。

Objective To establish a high-flow type of rat pulmonary hypertension model which can recapitulate clinical characteristics of pulmonary arterial hypertension in congenital heart disease with systemic-to-pulmonary shunts. Methods The animal model established by either left common carotid arter- y-external jugular vein shunts, or ligation of the left pulmonary artery, or combination of the two methods. Changes in mean pulmonary artery pressure （mPAP） , right ventricular hypertrophy index of right ventricle to left ventricle plus interventricular septum （ RV/LV ＋ S） , the ratio of vascular wall thickness to vascular external diameter （ WT% ） and the ratio of vascular wall area to total vascular area （ WA% ） were observed to compare different surgical approaches during postoperative 8 week. The changes of above indexes were also observed following combination of the two methods at 4, 8 and 12 week postoperation. Results As compared with control group, the mPAP and RV/LV ＋ S in combination of shunt and ligation of the left pulmonary artery group were significantly increased at 8th and 12th week （ P 〈 0. 01 ） ; Compared with con- trol group, the WT% and WA% also elevated obviously （P 〈 0. 01 ）. Conclusion Combination of shunt and ligation of the left pulmonary artery is an easy and reliable approach to create high flow-induced pulmo- nary arterial hypertension in rats, and can be used to study the pathogenesis of pulmonary arterial hyperten- sion caused by congenital heart diseases.