α1,3-FucT各亚型及SLeX在肝癌中的表达及意义

Expression and significance of α1, 3-FucT subtypes and SLeX in hepatocellular carcinoma

目的:检测α1,3-岩藻糖基转移酶(α1,3-FucT)各亚型及其修饰的糖链唾液酸路易斯糖X(SLeX)在肝癌细胞中的表达情况和相互关系。方法:采用Real-time PCR测定肝癌细胞系HepG2、SMMC-7721、HuH-7中α1,3-FucT各基因亚型的表达,流式细胞仪测定细胞表面总SLeX和核心2唾液酸路易糖X(core-2 SLeX)的表达情况。以正常肝细胞系HL-7702作为对照。结果:以正常肝细胞系HL-7702作为对照,肝癌细胞系中FUT6的表达升高是排他性的。各肝癌细胞系中,细胞表面总SLeX的表达无明显区别,但是core-2 SLeX在各细胞系之间区别明显。结论:α1,3-FucT在肝癌细胞系中以FUT6表达为主。不同细胞系表面SLeX抗原表达的差异可能为进一步研究肝癌转移复发提供有益思路。

Objective To detect the expression of α1, 3-FucT subtypes and its modified sugar chain X （SLeX） in hepatocellular carcinoma, and to study the mutual relations between α1, 3-FueT and SLeX. Methods Normal liver cell lines HL-7702 were compared with liver cancer cells HepG2, SMMC-7721, HUH-7. Expressions ofα1, 3-FucT in each genetic subtypes were determined by real-time PCR. Expression of cell surface sialic acid Louis sugar （SLeX） and core 2 sialic acid Louis sugar （core-2 SLeX） was measured by flow cytometry. Results Elevated FUT6 expression in hepatoma cell lines is exclusive as compared to normal liver cell lines HL-7702. The expression of cell surface SLeX was no significant difference in hepatoma cell lines, but there was a significant dill）fence in the expression of core-2 SLeX among these cell lines. Conclusion The expression of α1, 3-FucT genetic subtypes is cell-specific, where in FUT6 expression is mainly in hepatoma cell lines. Differential expression of surface SLeX atTtigen in different cell lines may provide a useful way of thinking about metastasis and recurrence of HCC.