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siRNA沉默RAD18抑制结肠癌SW480细胞FANCD2蛋白泛素化并增强其对丝裂霉素C的敏感性 被引量：4

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摘要 目的:研究RAD18特异性siRNA对结肠癌SW480细胞FANCD2泛素化和对化疗药物丝裂霉素C敏感性的影响。方法 :RAD18特异性siRNA转染SW480细胞后用丝裂霉素C处理,通过Western blot检测细胞RAD18及FANCD2泛素化的表达,MTT法检测干扰RAD18后细胞对丝裂霉素C的敏感性。结果:RAD18特异性siRNA转染SW480细胞96 h后,RAD18基因的表达明显降低,导致经丝裂霉素C处理后FANCD2泛素化表达明显降低;siRAD18组细胞对丝裂霉素C的敏感性显著增强。结论 :抑制RAD18蛋白表达能延缓结肠癌细胞FANCD2泛素化表达,并增强SW480细胞对丝裂霉素C的敏感性。
作者 钟雄平 陈业金 孔红祥 刘超 乐贻军 王红
机构地区 广州医学院附属广州市第一人民医院消化内科
出处 《实用医学杂志》 CAS 北大核心 2013年第11期1731-1733,共3页 The Journal of Practical Medicine
基金 2012年广州市科技计划项目(编号:2060404)
关键词 结肠肿瘤 RAD18 FANCD2 RNA干扰 SW480
分类号 R735.35 [医药卫生—肿瘤]
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参考文献17

  • 1Wang W. Emergence of a DNA-damage response network consisting of Fanconi anaemia and BRCA proteins [J]. Nat Rev Genet, 2007,8(10) :735-748.
  • 2Swift M, Fanconi's anaemia in the genetics of neoplasia [J]. Nature, 1971,230 : 370-373.
  • 3Wang H, Park H K, Zhang J, et al. Convergence of Rad6/ Radl8 and fanconi anemia tumor suppressor pathways upon DNA damage [J]. PLoS ONE, 2010,5(10):e13313.
  • 4Williams S A, Longerieh S, Sung P, et al. The E3 ubiquitin ligase RAD18 regulates ubiquitylation and chromatin loading of FANCD2 and FANCI[J]. Blood, 2011,117 (19) : 5078-5087.
  • 5Song I Y, Palle K, Gurkar A, et al. Radl8-mediated translesion synthesis of bulky DNA adduets is coupled to activation of the Fanconi anemia DNA repair pathway [J].J Biol Chem, 2010,285 (41) : 31525-31536.
  • 6Jemal A, Siegel R, WARD E, et al. Cancer statistics,2009 [J]. CA Cancer J Clin, 2009,59(4):225-249.
  • 7Giaechetti S, Perpoint B, Zidani R, et al. Phase III muhieenter randomized trial of oxaliplatin added to chronomodulated fluorouracil-leueovorin as first-line treatment of metastatic coloreetal cancer [J]. J Clin Oncol, 2000,18: 136-147.
  • 8Verdecchia A, Francisci S, Brenner H, et al. Recent cancer survival in Europe:a2000-02 period analysis of EUROCARE- 4data [J]. Lancet Oncol, 2007,8:784-796.
  • 9Levitus M, Joenje H, Winter J P. The Fanconi anemia pathway of genomic maintenance [J]. Cell Oncol, 2006,28 (1-2) :3-29.
  • 10Houghtaling S, Newel1 A, Akkari Y, et al. Fancd2 functions in a double strand break repair pathway that is distinct from non-homologous end joining [J]. Hum Mol Genet, 2005,14 (20) : 3027-3033.

同被引文献51

  • 1Marieke Levitus,Hans Joenje,Johan P. de Winter.The Fanconi anemia pathway of genomic maintenance[J]. Cellular Oncology . 2006 (1,2)
  • 2Williams Stacy A,Longerich Simonne,Sung Patrick,Vaziri Cyrus,Kupfer Gary M.The E3 ubiquitin ligase RAD18 regulates ubiquitylation and chromatin loading of FANCD2 and FANCI. Blood . 2011
  • 3Josephine C. Dorsman,Marieke Levitus,Davy Rockx,Martin A. Rooimans,Anneke B. Oostra,Anneke Haitjema,Sietske T. Bakker,J?rgen Steltenpool,Dezs? Schuler,Sheila Mohan,Detlev Schindler,Fré Arwert,Gerard Pals,Christopher G. Mathew,Quinten Waisfisz,Johan P. de Winter,Hans Joenje.Identification of the Fanconi anemia complementation group I gene, FANCI[J]. Cellular Oncology . 2007 (3)
  • 4Sato Koichi,Toda Kazue,Ishiai Masamichi,Takata Minoru,Kurumizaka Hitoshi.DNA robustly stimulates FANCD2 monoubiquitylation in the complex with FANCI. Nucleic Acids Research . 2012
  • 5Park Hwan Ki,Wang Hong,Zhang Jun,Datta Suvamoy,Fei Peiwen.Convergence of Rad6/Rad18 and Fanconi anemia tumor suppressor pathways upon DNA damage. PloS one . 2010
  • 6Wissmueller Sandra,Font Josep,Liew Chu Wai,Cram Edward,Schroeder Thilo,Turner Jeremy,Crossley Merlin,Mackay Joel P,Matthews Jacqueline M.Protein-protein interactions: analysis of a false positive GST pulldown result. Proteins . 2011
  • 7Masuda Y,Suzuki M,Kawai H,et al.Asymmetric na-ture of two subunits of RAD18,a RING-type ubiq-uitin ligase E3,in the human RAD6A-RAD18ternary complex. Nucleic Acids Research . 2012
  • 8Tsuji Y, Watanabe K, Araki K, et al. Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAI)6B protein triggers its recruitment to stalled replication forks [ J ]. Genes Ceils, 2008, 13 (4): 343-354.
  • 9Yamashita YM, Okada T, Matsusaka T, et al. RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells[J]. EMBO J, 2002, 21 (20) : 5558-5566.
  • 10Berwick M, Vineis P. Markers of DNA repair and susceptibility to cancer in humans: an epidemiologic review [J]. J Natl Cancer Inst, 2000, 92 (11) : 874-897.

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实用医学杂志
2013年 第11期

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