同源盒基因B7在肝癌组织中的表达及其临床意义

Expression and significance of homeobox B7 (HOXB7) in hepatocellular carcinoma

目的:检测肝癌组织中同源盒基因B7(HOXB7)的表达情况,探讨HOXB7的表达在肝癌发生发展中的意义。方法:分别用RT-PCR、Q-PCR和Western blot检测24例肝癌新鲜组织及相应癌旁正常组织中HOXB7 mRNA和蛋白表达水平的差异,用免疫组化法检测65例肝癌石蜡组织切片中HOXB7的表达,分析其与肝癌临床病理因素的关系。结果:HOXB7 mRNA和蛋白在肝癌组织中的表达水平高于癌旁正常组织。65例肝癌石蜡组织中,HOXB7强阳性率为52.3%(34/65)。HOXB7的强阳性与肝癌的TNM分期和病理分级密切相关,而与性别、年龄、肿瘤直径、HBsAg、肝硬化无明显相关性。结论:HOXB7在肝细胞肝癌中表达升高,HOXB7可成为评价肝癌发生、发展的生物学指标。

Objective To investigate the expression level and clinicopathologic significance of homeobox B7 （HOXB7） in hepatocellular carcinoma. Methods The expressions of HOXB7 mRNA and proteins were detected by RT-PCR, Q-PCR and Western blot in 24 samples of fresh hepatocellular carcinoma tissues and their normal adjacent tissues. Immunohistochemistry was used to examine the expressions of HOXB7 in 65 samples of paraffin-embedded hepatocellular carcinoma tissues. Results The expression levels of HOXB7 mRNA and proteins were significantly higher in the cancerous tissues than in the adjacent non-cancerous tissues. HOXB7 was strongly expressed in 52.3% （34/65） of the paraffin-embedded hepatocellular carcinoma tissues. Additionally, higher expression of HOXB7 was significantly associated with TNM classification and pathologic stages, while not with gender, age, tumor size, HbsAg, and liver cirrhosis. Conclusions HOXB7 is upregulated in hepatocarcinoma issues. HOXB7 may serve as a novel biomarker for predicting the pathogenesis and development of hepatocellular carcinoma.