摘要
目的:建立测定黄芩苷胶囊中黄芩苷含量的方法。方法:采用高效液相色谱法。色谱柱为Acclaim C18(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸溶液(47∶53,V/V),流速为1.0ml/min,检测波长为280nm,柱温为30℃。结果:黄芩苷的质量浓度在20.3~203.0μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.9999);精密度、重复性、稳定性试验的RSD均<2%;平均加样回收率为99.63%,RSD=0.313%(n=9)。结论:该方法准确、可靠、简单、快速,可用于黄芩苷胶囊的质量控制。
OBJECTIVE: To establish a method for the content determination of baicalin in Baicalin capsules. METHODS: HPLC method was adopted. The determination was performed on Acclaim C18(250mm×4.6mm,5μm) column with mobile phase consisted of methanol-0.1% phosphoric acid (47 : 53, V/V) at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm, and the column temperature was 30 ℃. RESULTS: The linear range of baicalin was 20.3-203.0 μg/ml (r=0.999 9) with an average recovery of 99.63 % (RSD=0.313%, n=6). The RSDs of precision test, reproducibility test and stability test were all low-er than 2 %. CONCLUSIONS: The method is accurate, reliable, simple and rapid. It is suitable for the quality control of Baicalin capsules.
出处
《中国药房》
CAS
CSCD
2013年第23期2182-2184,共3页
China Pharmacy