水盐浸种对NaCl胁迫下向日葵幼苗渗透调节物质的影响

Effects of Water and Salt Pretreatment on Osmoticregulation of Sunflower under NaCl Stress

以向日葵为材料,分别以水、400mmol/L NaCl浸种,评估二者对NaCl胁迫条件下(150mmol/L NaCl)向日葵幼苗中渗透调节物质积累的影响。结果表明:与对照相比,NaCl胁迫导致向日葵叶片、茎部脯氨酸(Pro)含量分别增加了3.36倍、2.40倍;经水、盐浸种后,向日葵叶片中Pro含量分别增加了6.19倍、4.72倍,茎部Pro含量差异不显著。与对照相比,NaCl胁迫处理不同程度地减少了向日葵叶片、茎、根中K+含量,增加Na+的吸收,K+:Na+明显降低;在NaCl胁迫条件下,水浸种、盐浸种不同程度地影响了幼苗中K+、Na+的含量、分配,但是经LSD比较,三种处理中离子含量没有明显差异。由此可见,在150mmol/LNaCl胁迫条件下,水、盐浸种更有效地诱导向日葵中Pro的积累来参与渗透调节。

The effects of pretreatment with water and 400mmol/L NaCI on the osmoregulation of the sunflower were investigated under 150mmoL/L NaCl stress. The results showed that： compared with the control treatment, the proline contents of leaves and shoots were increased by 3.36 times and 2.40 times, respectively. The proline contents of leaves pretreated by water and salt were increased by 6.19 times and 4.72 times, while there was no significant difference between the proline contents of shoot pretreated by water and salt. Moreover, K＋ content was decreased, Na＋ content was increased, and the ratio of K ＋ to Na ＋ was decreased under the salt stress. After pretreatment with water and salt, the contents and distributions of K ＋ and Na ＋ were affected in different degree. There was no signifi- cant difference among unpretreatment, water pretreatment and salt pretreatment. The results showed that organic os- moticum, especially proline induced by water and salt pretreatment, may played an important role in relieving the effect of salt stress.