摘要
为筛选水产用敌百虫的适用促渗剂,采用Franz扩散法,以杂交鳢[Channa maculate(♀)×Channa argus(♂)]的离体皮肤作为透皮屏障,研究了水溶性氮酮、丙二醇和薄荷醇对敌百虫体外经皮渗透的影响,采用气相色谱法(GC)测定各时间点接收液中敌百虫的浓度,通过计算在不同浓度促渗剂作用下敌百虫的平均累积渗透量(Q)、经皮吸收速率(Js)、渗透系数(KP)和增渗比(ER)等参数来比较渗透效果。结果显示:丙二醇和低浓度水溶性氮酮均具有促渗作用,其作用由强至弱依次为:体积分数为1×10-2的丙二醇>6×10-7的丙二醇>6×10-7的水溶性氮酮;而体积分数为1×10-2的水溶性氮酮对敌百虫的体外经皮渗透则表现出一定的拮抗作用;薄荷醇对敌百虫的经皮渗透无明显影响。
Experiments were conducted to study permeation-enhancing effect of various concentration of water soluble azone, propylene glycol and menthol on the in vitro percutaneous penetration of trichlorfon through hybrid snakehead skin [ Channa maculate ( if? ) x Channa argus ( 6 ) ], and selected the suitable penetration enhancers for trichlorfon used in aquaculture. Franz diffusion chambers were used and excised hybrid snakehead skin was chosen as transdermal barrier. GC method was established to determine the content of drug in receivers at specified time points. The in vitro permeation effects of every combination of trichlorfon with various concentration enhancers were evaluated through calculating and comparing the cumulative penetration amount of per unit area (Q), the rate of percutaneous absorption ( Js), permeability coefficient ( Ke ) and enhancement ratio ( ER ). The results revealed that propylene glycol and low concentrations of water soluble azone had the permeationenhancing effect,the sequence of effect was 1 × 10-2 propylene glycol 〉6 × 10-7 propylene glycol 〉 6 × 10 -7 water soluble azone(V/V). However, 1 × 10-2 water soluble azone(V/V) showed antagonism at certain times;1 mg/L and 2 mg/L menthol had little influence on the permeation-enhancing effect.
出处
《农药学学报》
CAS
CSCD
北大核心
2013年第3期305-310,共6页
Chinese Journal of Pesticide Science
基金
杭州市农业科研攻关专项(20120232B04)
关键词
促渗剂
水产
敌百虫
经皮渗透
penetration enhancers
aquaculture
trichlorfon
percutaneous penetration