响应曲面法优化微波辅提彩虹明樱蛤多糖的提取工艺

Optimization of Processing Parameters of Microwave-assisted Extraction Technique of Moerella iridescens Polysaccharide by Response Surface Methodology

目的:采用响应曲面法以微波辅助提取优化彩虹明樱蛤中多糖的提取工艺。方法:在单因素试验的基础上,以提取温度、提取时间、水料比为随机因子,多糖得率为响应值,进行3因素3水平的Box-Behnken中心组合设计,采用响应曲面法分析3个因素对响应值的影响并以此确定最佳提取工艺条件。结果:彩虹明樱蛤多糖的最佳提取工艺条件为:提取温度45℃,提取时间2.7 h,水料比50∶1,在此条件下多糖得率的验证值为1.17%,与预测值1.19%之间的相对误差为1.70%。结论:响应曲面法能较好地预测彩虹明樱蛤多糖的提取工艺,提高多糖提取率。

Objective：To optimize the extraction conditions of polysaccharide from Moerella iridescens with the microwave-assisted extraction technology by Response surface methodology（RSM）.Methods：Extracting temperature,extracting time and the solvent/solid ratio were selected as factors.The yield of polysaccharide was used as the responsive value.Based on the single factors experiments,a three-factor-three-level experiments design was developed by Box-Benhken central composite design method.The response surface method was employed to analyze the results of experiments.Results：The optimum extraction conditions of polysaccharide were as follows：extracting temperature was 45 ℃,extracting time was 2.7 h and solvent/solid ratio was 50∶ 1.The predictive maximum yield of polysaccharide was 1.17%.The average yield of polysaccharide in 3 validation experiments was 1.19% and the relative error was 1.70%.Conclusion：RSM analysis optimizing the extraction conditions for polysaccharide from Moerella iridescens is feasible,which can improve the extraction rate of Moerella iridescens polysaccharide.