藏药手掌参药材HPLC指纹图谱研究

Study on HPLC Fingerprint of Tibetan Herb Gymnadenia conopsea

目的:建立青藏高原不同地区藏药手掌参药材的HPLC指纹图谱。方法:采用反相高效液相色谱法,测得12批不同产地手掌参样品的指纹图谱。色谱条件:Kromasil C18(4.6 mm×250 mm,5μm)色谱柱,甲醇和水(含0.04%磷酸)梯度洗脱,流速0.7 mL/min,检测波长222 nm,柱温30℃。结果:建立了手掌参药材的HPLC指纹图谱,标定了13个共有指纹峰,方法学考察结果符合指纹图谱技术要求,12批手掌参药材的相似度为0.904～0.989;运用系统聚类分析法和主成分分析法对手掌参药材进行分类。结论:该方法简便、实用、可靠,为手掌参药材的鉴别和质量的全面控制提供了技术和理论依据。

Objective：To establish the HPLC fingerprint of Gymnadenia conopsea from different regions of Qinghai-Tibet Plateau.Methods：12 batches of Gymnadenia conopsea were measured by the RP-HPLC,and their fingerprints were obtained.Chromatographic condition： The sample was separated on column of Kromasil C18（4.6 mm×250 mm,5 μm） and gradiently eluted with a mixture of methanol and water（containing 0.04% phosphoric acid）.The flow rate was 0.7 mL/min.The detection wavelength was set at 222 nm and the column temperature was 30 ℃.Results：The HPLC fingerprint of Gymnadenia conopsea was set up and 13 common peaks were selected,the results of method validation met technical requirement of fingerprint,the similarity of 12 batches Gymnadenia conopsea was 0.904~0.989.Principal components analysis and clustering analysis were used in the identification of the samples.Conclusion：The method is simple,practicable and reliable,which can be used for the quality control of Gymnadenia conopsea.