不同药用部位茯苓UPLC-UV-MS指纹图谱比较研究

Study on UPLC-UV-MS Fingerprints of Different Medicinal Parts of Poria cocos

目的:建立并比较研究不同药用部位茯苓中三萜类成分的UPLC-UV-MS指纹图谱。方法:以去氢茯苓酸为对照品,采用Shim-pack XR-ODSⅡ(75 mm×2.0 mm,2.2μm)色谱柱,以乙腈-0.1%甲酸为流动相梯度洗脱;检测波长242 nm,流速为0.5 mL/min,柱温30℃;采用SPSS 16.0进行聚类分析。结果:建立茯苓中三萜类成分的UPLC-UV-MS指纹图谱,共标定22个峰,其中12个为共有峰,方法学考察结果符合指纹图谱技术要求;相似度和聚类分析结果表明,茯苓皮和茯苓块中三萜类成分存在显著差异,茯苓块药材质量与其产地有关,而茯苓皮药材不同产地间差别不大。结论:该研究方法稳定、可靠、重现性好,可用来控制茯苓的质量。

Objective： To establish an analytical method for the fingerprint of triterpenoid constituents of Poria cocos by UPLC-UV-MS and compare the fingerprints of different medicinal parts of Poria cocos.Methods： With dehydropachymic acid as reference substance,the separation was performed on Shim-pack XR-ODSⅡ（75 mm×2.0 mm,2.2 μm） analytical column.The mobile phase was consisted of acetonitrile and 0.1% formic acid as gradient eluent.The UV detection wavelength was 242 nm.The flow rate was 0.5 mL/min.The column temperature was 30 ℃.The cluster analysis was carried on by SPSS 16.0.Results： The UPLC-UV-MS fingerprints of triterpenoid constituents of Poria cocos were set up.The result showed that 22 peaks were found in different medicinal parts and 12 peaks of them were common.The results of method validation met technical requirement of fingerprints;Triterpenoid constituents in pared skin of Poria and peeled and sliced Poria were different,and the effect of habitat on the quality of peeled and sliced Poria was more obvious than that of pared skin of Poria.Conclusion： The method is stable and reliable with a good reproducibility and provides a reference standard for the quality control of Poria cocos.