牛角瓜花脂溶性成分及其抗菌活性研究

Liposoluble Constituents from the Flowers of Calotropis gigantea and Their Antimicrobial Activities

目的:研究牛角瓜Calotropis gigantea(L.)Dry.ex Ait.f.花脂溶性成分及其抗菌活性。方法:采用气相色谱-质谱(GC-MS)联用技术对牛角瓜花脂溶性成分进行分析,用面积归一化法计算各组分的相对含量;采用滤纸片琼脂扩散法测定其对2种人体病原菌的体外抑菌活性。结果:从牛角瓜花中共鉴定出31种脂溶性成分,占色谱总出峰面积的85.14%;牛角瓜花脂溶性成分对金色葡萄球菌(Staphylococcus aureus)、白色念珠菌(Canidia albi-cans)表现出一定抑制作用。结论:该研究为牛角瓜的开发利用提供了科学依据。

Objective： To study the liposoluble constituents from the flowers of Calotropis gigantea and their antimicrobial activities.Methods： The liposoluble constituents from the flowers of Calotropis gigantea were analyzed by GC-MS and their relative contents were determined by area normalization.Their antimicrobial activities against 2 pathogens were tested by agar disc diffusion method.Results： 31 compounds were separated and identified,amounting to 85.14% of the total constituents.The liposoluble constituents from the flowers of Calotropis gigantea exhibited weak antimicrobial activities against Staphylococcus aureus and Canidia albicans.Conclusion： The research result provides scientific foundation for exploitation and utilization of Calotropis gigantea.