大孔吸附树脂纯化百蕊草总黄酮工艺研究

Purification of Total Flavonoids from the Extracts of Thesium chinese with Macroporous Adsorption Resin

目的:研究D101大孔吸附树脂分离纯化百蕊草总黄酮的工艺条件。方法:以总黄酮转移率和质量分数为指标,采用动态试验考察D101大孔吸附树脂对百蕊草总黄酮的分离纯化效果及影响因素。结果:D101大孔吸附树脂对百蕊草总黄酮的最佳纯化工艺条件为:上柱液总黄酮浓度为2.259 mg/mL,上柱液速度为1 BV/h,洗脱剂为70%乙醇,洗脱速度为1 BV/h,洗脱剂量为4 BV。总黄酮转移率为94.44%,质量分数为12.45%,此质量分数较百蕊草浸膏中总黄酮的含量(2.91%)提高了4倍多。结论:D101大孔吸附树脂对百蕊草总黄酮有显著的富集作用,且该纯化工艺简单稳定。

Objective： To optimize the process parameters for purifying total flavonoids from Thesium chinese with D101 macroporous adsorption resin.Methods： Purification technology of total flavonoids from the extracts of Thesium chinese was investigated by dynamic adsorption method with the transfer rates and the purity of total flavonoids as indexes.Results： The optimal purifying conditions were as follows： sample concentration was 2.259mg / mL with a speed of 1 BV / h,eluent concentration was 70% alcohol with a speed of 1BV / h.After purification,the transfer rate of total flavonoids was 94.44% and the purity of total flavonoids achieved 12.45%,which was 4 times of that by coarse extraction（2.91%）.Conclusion： The purification technology is simple,stable and can significantly improve the contents of total flavoids in extracts.