IL-4基因多态性及幽门螺杆菌与柳州地区胃癌易感性研究

Correlation of IL-4 gene polymorphisms with Helicobacter pylori infection and susceptibility of gastric cancer in Liuzhou area,Guangxi

目的探讨白细胞介素4(Interleukin 4,IL-4)基因多态性和幽门螺杆菌(Helicobacter pylori,H.pylori)感染与广西柳州地区胃癌易感性的关系。方法采用前瞻性研究,用多聚酶链式反应(PCR)和半巢式聚合酶链式反应(Semi-nested PCR)对112例胃癌患者及238例慢性胃炎患者H.pylori的尿素酶B亚单位(UreB)基因和IL-4-588基因多态性进行分析。结果胃癌组H.pylori阳性率为59.8%,高于对照组的47.5%(P<0.05)。两组患者IL-4-588各基因型(T/T型、C/T和C/C型)分布频率差异无统计学意义(χ2=0.627,P=0.73);胃癌组含等位基因C、T者分别为的40例(17.9%)、184例(82.1%),其分布频率与对照组的73例(15.3%)、403例(84.7%)差异无统计学意义(χ2=0.715,P=0.398)。结论 IL-4-588基因多态性与H.pylori感染之间不存在交互作用,且与广西柳州地区胃癌易感性无关。

Objetive To explore the correlation of interleukin-4-588（IL-4-588）gene polymorphisms,Helicobaeter pylori（H.pylori） infection and susceptibility of gastric cancer in Liuzhou area of Guangxi Province.Methods The H.pylori urease B subunit（UreB） gene and IL-4-588 gene polymorphisms of 112 gastric cancer patients and 238 chronic gastritis patients were analyzedr using polymerase chain reaction（PCR） and semi-nested polymerase chain reaction（semi-nested PCR） method.Results H.pylori infection rates were 59.8% and 47.5% in gastric cancer group and control group,showing significant differences between the two groups（P0.05）.Differences in genotype frequencies of IL-4-588（T/T genetype、C/T genetype and C/C genetype） were not significant between two groups（χ2 = 0.627,P=0.73）;Distribution of allele frequency of C and T were 17.9% and 82.1% in gastric cancer group and that of the control group were 15.3% and 84.7%（χ2=0.715,P=0.398）.Conclusions There was no interaction between IL-4-588 gene polymorphism and H.pylori infection,and the gene polymorphism did not increase the susceptibility of gastric cancer in Liuzhou area of Guangxi Province.