APOBEC3F基因多态性与HBV感染易感性关系的探讨

Associations between APOBEC3G polymorphisms and hepatitis virus B infection

目的探讨载脂蛋白B mRNA编辑酶催化多肽3F(APOBEC3F)基因多态性与慢性乙型肝炎易感性的关系。方法提取179例HBV感染者及224例健康者外周血基因组DNA,采用PCR技术,扩增包含APOBEC3F第4外显子的片段,应用DNA测序技术对各组多态位点进行分型,计算各组等位基因频率。结果测序结果显示,APOBEC3F第四外显子-498位点、-532位点、-552位点在检测人群中不存在多态性,第-536位点基因发生T-C突变,两组差异有统计学意义(χ2=45.906,OR=0.260,95%C(I 0.173,0.391),P=0.000)。结论 APOBEC3F基因第4外显子-536位点T-C突变可能与HBV感染易感性有关。

Objective To evaluate the association of apolipoprotein B mRNA-editing enzymecatalyitc polypeptide-like 3F（APOBEC3F）polymorphism and hepatitis B virus（HBV） infection in population.Methods The genome DNA was obtained from blood samples and polymorphisms of APOBEC3F exon 4 were evaluated using PCR and Sanger in 179 HBV-infected patients and 224 healthy controls.Results No polymorphism at position-498、-532 and-552 was detected in all participants,but T-C mutation at position-536 was observed showing significant difference between the hepatitis B cases and controls（χ2=45.906,OR=0.260,95%CI（0.173,0.391）,P=0.000）The allele was found only in T/C genotypes.Conclusions The T-C mutation at position-536 of APOBEC3F exon 4 might be associated with the susceptibility to HBV infection in Han population in southern China.