白条草蜥多态性微卫星位点的分离与鉴定

Isolation and Characterization of Nine Polymorphic Microsatellites in White-striped Grass Lizard

白条草蜥(Takydromus wolteri)是一种年产多窝卵的蜥蜴。为了对其婚配制度、同一雌性个体所产卵的窝内和窝间的父权状况、种群的遗传结构和物种的进化历史等研究内容进行探讨,本研究筛选出白条草蜥的9个具有高度多态性的微卫星位点。微卫星位点筛选自包含(AC)n和(ATAG)n重复片度的微卫星富集文库。在白条草蜥安徽滁州种群的16～32个个体中对上述位点进行检测后发现,上述座位的等位基因数目范围为12～20个,期望杂合度范围为0.894～0.955,观测杂合度范围0.565～0.938,表明这些微卫星标记具有良好的遗传多样性,它们将在白条草蜥的种群遗传结构、基因流水平、种群分化和婚配制度的研究中发挥重要作用。

White-striped Grass Lizard （Takydromus wolteri） is an oviparous lacertid lizard. Females produce multiple clutches of eggs during the breeding season. To understand the mating system, the paternity of the offspring within and among clutches, the population genetic structure and the evolutionary history of this species, we developed 9 highly polymorphic microsatellites for T. wolteri in a population from Chuzhou, Anhui, China. Loci were isolated from a genomic library enriched for （AC） o and （ATAG） n repetitive elements and primers were tested in 16 - 32 individuals. The number of alleles ranged from 12 to 20 per locus, the expected heterozygosity （He） ranged from 0. 894 to 0. 955, and the observed heterozygosity （Ho） ranged from 0. 565 to 0. 938. These loci could be useful markers to investigate population gen.etic structure, gene flow level, population differentiation and mating systems in T. wolteri.