大肠癌中αvβ3 integrin表达与血管生成拟态的关系及分子机制的研究

Relationship between αvβ3 integrin and vasculogenic mimicry expression and related molecular mechanisms

目的:探讨大肠癌中是否存在血管生成拟态(VM),阐述VM存在的临床意义;分析VM与αvβ3 integrin表达的关系;研究大肠癌中αvβ3 integrin表达的临床意义及其与FAK、MMP-2、VEGF表达的相关性。方法:对227例大肠癌组织切片进行CD31免疫组织化学和PAS染色及αvβ3 integrin、FAK、VEGF、MMP-2免疫组织化学染色。结果:VM在大肠癌中阳性率为18.06%(41/227),αvβ3 integrin在大肠癌中阳性率为56.83%(129/227);VM阳性组、αvβ3 integrin阳性组生存时间均较阴性组短,差异均有统计学意义(P<0.05);VM阳性组的αvβ3 integrin阳性率较阴性组高,差异有统计学意义(P<0.05);αvβ3 integrin与FAK、VEGF、MMP-2表达呈正相关。结论:大肠癌中VM的存在、αvβ3 integrin的表达与肿瘤的不良预后有关;αvβ3 integrin可能通过与FAK、VEGF、MMP-2的相互作用,参与了大肠癌VM的形成。

Objective： This work aimed to investigate the expression and clinical significance of vasculogenic mimicry （VM）, to analyze the relationship between VM and αvβ3 integrin expressions, and to study the clinical significance of αvβ3 integrin expression and its correlation with the Focal Adhesion Kinase （FAK）, Vascular Endothelial Growth Factor （VEGF）, and Matrix Metalloproteinase 2 （MMP-2） expression. Methods： VM expression was observed by CD31 immunohistochemical and PAS staining. Expression of αvβ3 integrin, FAK, VEGF, and MMP-2 was observed by immunohistochemical staining. Results： Results showed that VM and av133 integ- rin had positive rates of 18,06% （41/227） and 56.83% （129/227）, respectively. Patients with VM expression had a shorter survival time than those without VM expression （P〈0.05）.αvβ3 integrin-positive patients had a shorter survival time than αvβ3 integrin-negative pa- tients （P〈0.05）. Patients with VM expression showed a higher positive rate of αvβ3 integrin than those without VM expression （P〈 0.05）, and αvβ3 integrin had a positive correlation with FAK, MMP-2, and VEGF expression. Conclusion： The incidence of VM and αvβ3 integrin expressions in human colorectal cancer cases indicates a poor prognosis, αvβ3 integrin possibly participates in the formation of VM through interactions with FAK, VEGF, and MMP-2.