盐酸表柔比星联合奥沙利铂对肝癌HepG2细胞凋亡的抑制作用

Inhibitory effect of epirubcin hydrochloride co-treated with oxaliplatin in the apoptosis of HepG2 cells

目的:探讨盐酸表柔比星联合奥沙利铂对肝癌细胞HepG2的抑制作用。方法:盐酸表柔比星和奥沙利铂单独及联合给药用MTT法测定HepG2细胞的增殖,用Hoechst 33258染色法检测细胞核凋亡情况,用DNA ladder检测DNA的裂解情况。结果:人肝癌HepG2细胞经奥沙利铂、盐酸表柔比星以及两者联合作用后,在不同时间、不同浓度均能出现细胞抑制作用,并且均呈现剂量-时间依赖效应,与单药组相比,联合用药组抑制率明显增高,q值大部分为0.85～1.15,呈现明显的相加作用,在(35+5)μg/mL组作用24h,(20+2)μg/mL组作用48、72h时两组实验组q值<0.85,出现拮抗作用。(25+3)μg/mL组作用24h为最佳作用时间和作用浓度。结论:奥沙利铂及盐酸表柔比星对肝癌HepG2细胞有增殖抑制及促凋亡作用,两药联合作用表现为相加作用,其机制需进一步研究。

AIM：To investigate the inhibitory effect of epirubcin hydrochloride in combination use with oxaliplatin（L-OHP） on the hepatocellular carcinoma cells HepG2.METHODS：The inhibitory effect of epirubicin hydrochloride together with L-OHP on the growth of human hepatic carcinoma cell line HepG2 in vitro was evaluated by MTT assay.The apoptosis of nucleus was detected by Hoechst33258staining.The degradation of DNA was evaluated by DNA ladder.RESULTS：Oxaliplatin and epirubicin hydrochloride both inhibited the proliferation of HepG2cells,it presented addictive inhibition effect when two medicine combined（q value was in 0.85-1.15）.CONCLUSION：Oxaliplatin and epirubicin hydrochloride both inhibited the proliferation of HepG2cells and promoted apoptosis,combination of these medicine presented addictive inhibition effect,however,the mechanism was unclear.