γ-生育三烯酚对人胃癌细胞转移潜能影响及其机制的探讨

Effects of γ-tocotrienol on migration and invasion of gastric cancer cells and its mechanisms

目的:探讨γ-生育三烯酚(γ-T3)对人胃癌细胞(SGC-7901和MGC-803)迁移和侵袭能力的影响。方法:不同浓度的γ-T3对SGC-7901和MGC-803细胞作用24h后,采用CCK-8法、迁移试验、Transwell小室侵袭试验和酶联免疫吸附试验(ELISA)研究γ-T3对细胞增殖、迁移能力和侵袭能力,以及基质金属酶(MMPs)表达量的影响。结果:CCK-8的结果显示,γ-T3对SGC-7901和MGC-803细胞有抑制作用,并具有剂量-效应关系;体外划痕试验的结果显示,随着药物浓度增加,迁移至划痕区的细胞逐渐减少,60μmol/L组仅有少许细胞迁移至划痕区。在MGC-803细胞中F=25.55,P=0.00;在SGC-7901细胞F=36.15,P=0.00,差异有统计学意义;Transwells小室侵袭实验结果显示,肿瘤细胞侵袭穿透Matrigel胶和Transwell小室微孔膜的能力明显下降,穿膜细胞数目随着药物浓度增加明显减少,在MGC-803细胞中F=23.53,P=0.00;在SGC-7901细胞F=26.00,P=0.00,差异均有统计学意义;ELISA的结果显示,细胞培养上清液中MMP-2和MMP-9分泌量随药物浓度增加而降低。在MGC-803细胞MMP-2中F=62.41,P=0.00;MMP-9中F=314.18,P=0.00。在SGC-7901细胞MMP-2中F=52.58,P=0.00;MMP-9中F=70.00,P=0.00,差异均有统计学意义。结论:γ-T3可显著抑制SGC-7901和MGC-803细胞的增殖、迁移和侵袭,其机制可能与选择性抑制SGC-7901和MGC-803细胞分泌MMP-2和MMP-9有关。

OBJECTIVE： To evaluate the effects of the 7-tocotrienol （Y--T3） on the migration and invasion of SGC-7901 and MGC-803 cell lines. METHODS： Different concentrations of Y-T3 on SGC-7901 and MGC-803 cells cultured after 24 h, CCK-8 assay, scratch wound assay and Transwell chamber in vitro invasion assay were used to detect the change of proliferation, migration and invasion of cells. The levels of MMP-2 and MMP-9 were evaluated by enzyme- linked immunosorbent assy（ELISA）. RESULTS： CCK-8 results showed：Y-T3 inhibition of SGC-7901 and MGC-803 cells in a dose-effect relationship; The scratch test in vitro results showed that the cells that migrated to the scratch area de- creased with increasing drug concentration,60 amol/L group was just a few cells to migrate to the scratch area. MGC-803 cells showed （F=25.55 ,P=0.00） and SGC-7901 cells showed （F=36.15 ,P=0.00） ,the difference was statistically sig- nificant; Transwells chamber invasion assay results showed that：the invasion capacity of tumor cells in penetrating Matri- gel gel and Transwell chamber microporous membrane decreased and the number of cell penetrating significantly reduced with the increase in drug concentration in the MGC-803 cells （F = 23.53, P=0.00）; SGC-7901 cells （F=26.00, P = 0.00） ,the difference was statistically significant; ELISA results showed that：the cell culture supernatant of MMP-2 and MMP-9 secretion was decreased following drug concentration increasing. MGC-803 cells, MMP-2 （F= 62.41,P= 0.00）, MMP-9 （F=314.18,P=0.00） ; in SGC-7901 cells MMP-2 （F=52.58,P=0.00）,MMP-9 （F=70.00,P=0.00）,the difference was statistically significant. CONCLUSION： The Y-T3 can significantly inhibit the proliferation,migration and invasion of SGC-7901 and MGC-803 cell lines through down regulating the levels of MMP-2 and MMP-9.