乳腺癌组织HSG和EGFR基因mRNA表达与临床病理因素相关性分析

HSG and EGFR mRNA expression in breast cancer and its relation to clinicopathological features

目的:研究增殖抑制基因(HSG)和表皮生长因子受体(EGFR)在乳腺癌中的表达及其与临床病理因素的相关性。方法:应用实时荧光定量PCR及免疫组织化学方法来检测HSG和EGFR的mRNA在正常乳腺组织、乳腺纤维腺瘤和乳腺癌组织中的表达,以及它们在乳腺癌组织中表达的相关性。结果:乳腺癌组HSG mRNA表达水平和表达率显著低于正常乳腺组和乳腺腺瘤组,P<0.05。乳腺癌组EGFR mRNA表达水平和表达率显著高于正常乳腺组和乳腺腺瘤组,P=0.000。乳腺纤维腺瘤组织HSG和EGFR mRNA表达水平和阳性检出率与正常乳腺组织比较差异无统计学意义,P>0.05。乳腺癌组织HSG和EGFR的mRNA表达与淋巴结转移和病理组织学分级以及雌激素受体(ER)和HER-2有关,而与肿瘤类型、肿瘤大小、临床分期和年龄等无明显相关性,P>0.05。HSG与EGFR的mRNA表达负相关,r=-0.456,P=0.002。结论:乳腺癌组织HSG的mRNA表达随着分化程度的下降或淋巴结的转移或ER和HER-2阴性者,HSG的表达明显下降。而EGFR mRNA在组织分化程度低或ER和HER-2阳性的乳腺癌中的表达却明显增高。乳腺癌组织HSG的表达与EGFR的表达呈负相关。HSG与抑制乳腺癌组织的发生和淋巴转移正相关。

OBJECTIVE：To study the expression of hyperplasic suppress gene （HSG）,epidermal growth factor re- ceptor （EGFR） and their correlation in breast cancer tissues. METHODS： The method of real-time Fluorescene quantita- tive polymerase chain reaction（RT-PCR） were applied to detect the proliferation of HSG. EGFR mRNA and the protein expression of HSG, EGFR were detected by using SP immunohistochemistry in normal breast tissue, breast carcinoma, breast fibroadenoma tissues and their relevance in breast cancer tissues. RESULTS： The expression of HSG mRNA was significantly lower in breast cancer than those in breast fibroadenoma and normal breast tissue （P〈0.05）; The expres- sion of EGFR mRNA was significantly higher in breast cancer than those in breast fibroadenoma and normal breast tissue （P〈0.05） ; The expression of HSG mRNA and EGFR mRNA in breast fibroadenoma and normal breast tissues showed no statistically significant difference （P〉0.05）. The expression of HSG and EGFR mRNA was correlated with lymph node metastasis,different pathological grades of breast cancer, ER and HER-2 and that was incorrelated with the age of the patient,tumor type,tumor size and clinical stage （P〉0.05）. The expressions of HSG and EGFR were negative corre- lation in breast cancer tissues（r= --0. 456, P= 0. 002）. CONCLUSIONS： With the decline in the degree of differentiation or lymph node metastasis and the negative expression of ER and HER-2, the expression of HSG mRNA decreased signifi- cantly in breast cancer tissues. The expression level of EGFR is significantly increased in low pathological grades and the positive expression of ER and HER - 2 of breast cancer. The expression of HSG is negatively correlated with EGFR inbreast cancer tissues. The expression of HSG is positively correlated with inhibitting the occurrence of cancer tissue and the metastasis of lymph node.