摘要
目的:研究大蒜素对人食管癌EC9706细胞凋亡的诱导作用。方法:0、18g/mL大蒜素处理人食管癌EC9706细胞,应用细胞计数、流式细胞仪、Hoechst33258染色、HE染色和电镜观察经大蒜素诱导处理后EC9706细胞的凋亡情况。结果:与对照组比较,经18μg/mL大蒜素诱导处理7d后,EC9706细胞的增殖活动受到明显抑制,细胞生长抑制率达75.89%(P<0.05);细胞周期检测出现亚二倍体(亚G1期)细胞峰值,细胞凋亡率达23.8%(P<0.05),并发生G2/M期阻滞;经Hoechst33258染色出现细胞核浓染及致密的固缩形态和颗粒状荧光;光镜和电镜观察结果均显示,大蒜素处理组细胞体积缩小,细胞核固缩,出现染色质凝聚、边集化等显著的凋亡特征。结论:大蒜素对人食管癌EC9706细胞具有凋亡诱导作用。
OBJECTIVE: To study the apoptotic effect of garlicin on esophageal cancer EC9706 cells. METHODS: We used 18 μg/mL garlicin to treat esophageal cancer EC9706 cells,and analyzed the effect by cell culture,cell count,flow cytometry analysis,Hoechst33258 staining,HE staining and electron microscopy. RESULTS:Proliferation of EC9706 cells could be inhibited by 18 μg/mL garlic,cell growth inhibition rate was 75.89%. Flow cytometry analysis showed that garlic could induce the emergence of apoptosis,and the rate was 23.8%,blocked at G2/M phase. Concentrated cell nucleus appeared granular and fluorescent by Hoechst33258 staining. Light microscope and electron microscope showed that the morphology of the cells treated with 18 μg/mL garlic appeared shrunken,cell nucleus concentrated,chromatin agglutination,mitochondria swelling,and other significant apoptosis characteristics. CONCLUSION:This study suggested that garlic could induce apoptosis of esophageal cancer EC9706 cells effectively.
出处
《癌变.畸变.突变》
CAS
CSCD
2013年第3期201-204,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
河南省重点科技攻关项目(092102310006)
河南省教育厅基金项目(2009B180001)