骨髓间充质干细胞移植对缺血性脑损伤大鼠的治疗作用

Effect of bone marrow mesenchymal stem cell transplantation in the treatment of ischemic brain injury in rats.

目的研究骨髓间充质干细胞(BMSCs)移植对大鼠缺血缺氧性脑损伤(HIBD)的治疗作用。方法选取15只SD大鼠均分三组,模型组、假手术组和BMSCs组各5只,并统一行左颈总动脉分离,行双层结扎制作大鼠缺血性脑损伤模型,另选150只雄性大鼠,取其骨髓液,在经离心、取悬液、接种培养后制备BMSCs悬液,将细胞数约为1×105个的BMSCs悬液注入各组大鼠的头骨中,灌流处死大鼠,取脑处理并对脑组织切片行HE染色和免疫荧光染色,观察其病理特征。结果三组脑组织结构形态略有出入,模型组脑中发现大量坏死神经元,BMSCs组脑组织情形介于上述两组之间。移植BMSCs4周后,该组脑神经元坏死指数为(0.170±0.0003),假手术组指数为(0.094±0.0002),两组比较差异无统计学意义(P>0.05),上述两组同模型组(0.342±0.0003)比较,差异有统计学意义(P<0.05)。结论移植BMSCs能有效降低大鼠脑内神经元坏死率,有助于神经系统复原,且能够有效治疗缺血缺氧性脑损伤。

Objective To study the effect of bone marrow mesenchymal stem cells （BMSCs） transplantation in the treatment of hypoxic-ischemic brain damage （HIBD） in rats. Methods Fifteen SD rats were selected and divided equally into three groups： model group, sham operation group and BMSCs group, each with 5 cases. The rats underwent separation of the left common carotid artery and double ligation to establish ischemic brain damage rat model. The bone marrow of another 150 male rats were collected, centrifuged, suspended, and then cultured to prepare BMSCs suspension. The BMSCs suspension （with approximately 1×10^-5 cells） was then injected into the skull of the rats in each group. The rats were then killed. The brain tissue was taken and stained by HE staining and immunofluorescence staining to observe the pathological features. Results The structure and morphology of brain tissue in the three group is slightly different. A large number of necrosis of neurons were found in the brain in model group, and the neerosis of neurons in BMSCs group was between the other two groups. Four weeks after the transplantation of BMSCs, the index of necrosis of neurons was （0.170±0.0003） in BMSCs group and （0.094±0.0002） in sham operation group, which had no statistically significant difference between the two groups （P〉0.05）, but showed statistically significant difference with that of the model group （0.342±0.0003）, P〈0.05. Conclusion Transplantation of BMSCs can effectively reduce the rate of neuronal necrosis in rat brain, which helps recover the nervous system and effectively treat hypoxic-ischemic brain damage.