结核患者外周血中辅助性T细胞17及白细胞介素-17水平的变化

Levels of Th17 cells and IL-17 in tuberculosis patient's peripheral blood

目的:探讨结核患者外周血辅助性T细胞17(Th17细胞)及白细胞介素-17(IL-17)水平变化及意义。方法:以结核患者98例(初治58例,复治40例)、健康对照者98例为研究对象,抽取研究对象外周血并分离外周血单核细胞(PBMC)和血浆,采用流式细胞术(FCM)检测PBMC中Th17细胞的百分率,酶联免疫吸试验(ELISA)检测血浆中IL-17水平;用灭活的结核分枝杆菌菌株(H37Rv)分别刺激结核患者和健康对照者PBMC,ELISA法检测其上清中IL-17水平。结果:与健康对照者比较,结核患者外周血Th17细胞比例[(5.2±1.8)%vs(1.8±1.4)%,P<0.05]及IL-17水平[(68.22±10.31)vs(39.81±14.58)pg/ml,P<0.01]显著增高;与初治组比较,复治组Th17细胞比例[(6.4±1.8)%vs(3.8±1.3)%,P<0.05]及IL-17水平[(85.46±9.13)vs(51.22±12.36)pg/ml,P<0.05]显著增高;结核患者血浆IL-17水平与Th17细胞的比例呈正相关(r=0.912,P<0.05);H37RV刺激结核病人和健康对照者PBMC后上清中均有IL-17的表达,且结核病人来源的表达水平更高。结论:结核患者外周血Th17细胞比例及IL-17水平增高,Th17细胞和IL-17在结核病的发生发展中发挥了重要作用。

Objective： To investigate the changes and clinical significance of Th17 cells and IL-17 levels in tuberculosis （TB） patient＇s peripheral blood. Methods： The study involved 98 TB patients （58 initial treat- ment patients, 40 re-treatment patients） and 98 healthy controls. Peripheral blood mononuclear cell （PBMC） and plasma derived from their peripheral blood were tested. Flow cytometry was used to determine the per- centage of Th17 cells in PBMC, enzyme-linked immunosorbent assay （ELISA） was used to determine the levels of IL-17 in plasma;TB patients and healthy controls＇ PBMC were infected with heat-inactivated myco- bacterium tuberculosis （M. th） H37Rv, the levels of IL-17 in supernatant of PBMCs were measured by ELISA. Results： To compare with the healthy controls, the percentages of Th17 cells in TB patients＇ periph-eral blood were significantly increased [（5.2±1.8）% vs（1.8±1.4）%, P〈0. 05-]; the levels of IL-17 in TB patient＇s plasma were significantly higher than that in healthy controls [ （68. 22 ± 10. 31）vs （39. 81±14. 58） ng/L, P〈0. 011. In addition, compared with initial treatment patients, the percentages of Th17 cells [（6.4±1.8）% vs（3. 8±1. 3）%, P〈0. 051 and IL-17 levels [（85.46±9. 13）vs （51.22±12. 36）ng/L, P〈0. 05] in re-treatment patients were significantly increased. The levels of IL-17 and the percentages of Th17 cells were positively correlated（r=0. 912,P〈0. 05） ;IL-17 in the supernatant of TB patients and healthy controls＇ PBMC infected with M. tb H37Rv were expressed, relatively high levels were from TB patients＂ supernatant. Conclusion. The percentages of Th17 cells and the levels of IL-17 in TB patienfs peripheral blood are signifi- cantly increased compared with healthy controls, Th17 cells and IL-17 play important roles in the initiation and progression of TB.