血清TGF β1,Ⅳ型胶原,层粘连蛋白与糖尿病肾病的关系

Association of Serum Transforming Growth Factor-(TGF--β1), Type Ⅳ Collagen and Laminin with Diabetic Nephropathy in Type 2 Diabetic Patients

目的:研究2型糖尿病患者血清TGF-β1,Ⅳ型胶原,层粘连蛋白水平与糖尿病肾病之间的关系和对早期糖尿病肾病的诊断意义。方法:应用ELASA法测定61例2型糖尿病患者和18例健康者的血清Ⅳ型胶原,层粘连蛋白,血清TGF-β1水平。2型糖尿病患者根据尿蛋白排泄率(UAER)分为UAER正常组和糖尿病肾病组。糖尿病肾病组进一步根据UAER分为微量蛋白尿组和临床肾病组。结果用SPSS12.0统计学软件做统计学分析。结果:糖尿病患者的血清TGF-β1,Ⅳ型胶原,层粘连蛋白水平高于正常对照组(P<0.01);糖尿病患者各组的血清Ⅳ型胶原和层粘连蛋白水平随UAER增高依次增加(P<0.01);糖尿病各组间血清TGF-β1水平无明显差异(P>0.05)。结论:2型糖尿病患者血清Ⅳ型胶原,层粘连蛋白,TGF-β1水平均高于正常人,血清Ⅳ型胶原,层粘连蛋白,TGF-β1与DN的发生,发展有密切的关系,血清Ⅳ型胶原可以作为诊断早期糖尿病肾病的指标,血清Ⅳ型胶原还是反映糖尿病肾病严重程度的重要预测指标。

Objective： To investigate the relationship between serum level of type Ⅳ collagen, Laminin, TGF- β1 and diabetic nephropathy in type2 diabetic patients Moreover, Investigate the Significance in early diagnosis of diabetic nephropathy. Methods： Serum levels of type IVcollagen ,laminin （LAN）, and TG-F-β 1 were determined with ELISA method in 61 patients with type 2 diabetes melli-tus and 18 nor-mal controls. Diabetic patients were divided into patients with diabetic nephropathy （DN） and patients without DN （UAER〈30mg/24h） according to the urine albumin elu-tion rate（UAER）. Moreover, patients with DN were divided into patients with mi-cro album in urine （MA,（UAER 30-300mg/24h） and patients with clinical DN （UAER〉300mg24h）.The data were analysised by SPSS （Statistical Program for Social Sciences） 12.0 software. Results：In both patients with DN and patients without DN, serum level of type Ⅳ collagen, LAN were higher than those in normal subject. （P〈0.01）.The difference of serum level of type Ⅳ collagen and LAN between di-abetic patients is significant. （P〈0.01） Moreover, serum level of type Ⅳ collagen and LAN between diabetic patients increase according to UAER. There were no differences when Serum TGF β 1 was compared between diabetic patients. （P〉0.05） Conclusion：Seru-m type IV collagen, TGF β 1 and LAN were increased in patients with type2 diabetes as compared with normal subjects, and had the close re-lationship with the happiness and development of diabetic nephropathy （DN）. Serum type Ⅳ collagen can be used as index of early DN. Serum type IV collagen appears to be the predictor of severity in DN.