银杏叶提取物总黄酮的提取分离及工艺的研究

Study on the Extraction and Separation of Total Flavonoids from Ginkgo Biloba L. Extract

本文首先是银杏叶提取物的制备,我们采用70%乙醇热回流提取工艺提取银杏叶,用紫外可见分光光度法和高效液相色谱法对提取物中的银杏叶总黄酮进行含量测定,并相互比较。选择一种快速、简便、准确的方法,作为银杏叶总黄酮含量测定的常用方法,并加以推广应用。同时以高效液相色谱法为测量手段,以总黄酮的收率为指标,对银杏叶的提取与精制工艺进行研究和探讨,确定了提取溶剂、时间、次数及浓缩、干燥温度等提取工艺参数,比较了树脂吸附一次洗脱和二次洗脱以及超滤与树脂吸附相结合的精制工艺对产品质量及银杏叶总黄酮收率的影响。结果满意。

The Ginkgo biloba L. extract were obtained through the method of 70% ethanol thermal recycling extract from G. biloba leaves. We determined the total flavonoids content in the leaves of G. bilaba by ultraviolet-visible spectroscopy and high performance liquid chromatography （HPLC） to find the most appropriate method, which could be considered as a regular method for determining the total flavonoids content. The extraction and separation process of total flavonoids in G biloba was investigated using HPLC, and the process parameters such as extraction reagents, time as well as condensing and drying temperatures and so on were optimized to acquire higher recoveries. We also compared the effects of resin adsorption as well as the combination of ultrafihration and resin adsorption on the quality and recovery of total flavonoids in G. biloba.