甲胺磷生物降解酶对韭菜中农残生物修复效果研究

Bioremediation from Enzyme of Methamidophos Pesticide Degrading Bacteria to Chives

利用大庆碱性寒地黑土分离得到的一株葡萄球菌XY-C,采用含甲胺磷的液态培养基对该菌株代谢产酶。通过单因素确定该酶对韭菜中甲胺磷农药残留的降解工艺参数,以甲胺磷降解率作为评价指标。获得了粗酶液对韭菜中甲胺磷农药降解的最佳降解条件为:降解温度45℃,酶液添加量20mL,分别喷洒3次,于45℃降解反应20min,采用最优降解条件,粗酶液对韭菜中甲胺磷农药的实际降解率达到67.17±1.17%。

The research uses a Staphylococcus Rosehbach XY-C separated in the alkaline cold black soil in Daqing. Liquid culture is used to make Staphylococcus Rosehbach XY-C produce methamidophos dgrada- tion enzyme. Degradation technological parameters of the coarse enzyme to methamidophos pesticide resi- dues are determined according to single factor test. The technological parameters use degradation ratio of methamidophos as evaluation indicator. Eased on the experimental data, the optimum degradation condi- tions of coarse enzyme to the biodegradation of methamidophos in chives are obtained. The optimum con- ditions are： optimal temperature is 45℃, optimal enzyme dosage is 20mL, spray three times respective and optimal reaction time is 20min. Degradation ratio of coarse enzyme to methamidophos pesticide resi- dues was 67.17±1.17% based on the optimum degradation conditions.