23株酵母菌株遗传亲缘关系的SRAP分析

Analysis of the Genetic Relations of 23 Yeast Strains by SRAP Markers

探讨酵母菌株间的遗传亲缘关系以及遗传多样性,为酵母菌株的分类和鉴定提供科学依据。利用44个SRAP(sequence-related amplified polymorphism)引物组合对23株酿酒酵母菌株进行聚类分析、主成分分析及遗传多样性的评价。结果表明,从160个SRAP引物组合中筛选得到44个多态性引物组合,共检测到529条DNA带,其中多态性条带有465个,多态性条带百分率为86.20%,平均每个引物组合产生多态性条带10.1个。各菌株遗传相似系数在0.531～0.813之间,平均遗传相似系数为0.671;根据UPGMA聚类分析,23株酵母菌种在遗传相似系数0.610处被分为两大类群,在遗传相似系数0.705处被进一步划分为6个亚类群。第Ⅰ大类又分为Ⅰa(包括1、2、4、7、10、11、12、14和13号酵母菌株)、Ⅰb(包括15、16、19、20和21号酵母菌株)、Ⅰc(包括17和23号酵母菌株)及Ⅰd(18号酵母菌株);第Ⅱ大类分为Ⅱa(包括3、8、9和6号酵母菌株)和Ⅱb(包括5和22号酵母菌株)。主成分(PCA)分析结果与此一致。这也与形态及生理特性的分类基本相似。SRAP标记技术能很好地用于酿酒酵母遗传亲缘关系、菌种鉴定和分类的研究,是一种经济、有效和可靠的分子标记技术。

In this study,the genetic relations and genetic diversity among 23 Saccharomyces cerevisiae strains were investigated by using sequence-related amplified polymorphism（SRAP） markers to provide scientific data for the classification and the identification of Saccharomyces cerevisiae strains.UPGMA cluster analysis,principal coordinate analysis（PCA） and genetic diversity analysis of 23 S.cerevisiae strains were carried out by using 44 SRAP primer combinations.The results showed that 44 SRAP primer combinations generated a total of 529 scorable bands,with an average of 11.6 bands per primer pair,of which 465（86.20 %） were polymorphic.The average number of polymorphic bands amplified by per primer combination was 10.1.The genetic similarity coefficient among all strains varied from 0.531 to 0.813 with the average coefficient as 0.671.UPGMA method cluster analysis showed that 23 strains were classified into two main cluster groups with the genetic similarity coefficient of 0.610 and further divided into six subgroups with the genetic similarity coefficient of 0.705.Group I was further divided into four subgroups： group Ia（including No.1,2,4,7,10,11,12,14 and 13 strain）,group Ib（including No.15,16,19,20 and 21）,groupⅠc（including No.17 and 23 strain） and groupⅠd（including 18 strain）.Group II was also divided into two subgroups： groupⅡa（including No.3,8,9 and 6 strain） and groupⅡb（including No.5 and 22 strain）.Principal coordinate analysis（PCA） results were almost the same and the results were also consistent with morphological and physiological data of these strains.SRAP markers could be used for the study of genetic relations of S.cerevisiae strains and their classification and identification and it was a simple,efficient and reliable method.