高速逆流色谱分离纯化木芙蓉叶中芸香苷

Separation and Purification of Rutoside from Hibiscus Mutabilis Leaves Using High-speed Counter-current Chromatography

目的建立高速逆流色谱(HSCCC)分离纯化木芙蓉叶中芸香苷的方法。方法采用水提取法制备芙蓉叶粗提物,应用大孔吸附树脂以60%乙醇为洗脱液对粗提物进行洗脱,达到去除杂质和获得黄酮类物质的目的,应用高速逆流色谱仪,以乙酸乙酯∶正丁醇∶水(1∶0.3∶1.5)上相为固定相,下相为流动相,组成溶剂体系对芙蓉叶提取物进行分离,从而获得高纯度的芸香苷。结果经对提取的芸香苷粗提物及HSCCC法纯化后的峰样品进行高效液相色谱(HPLC)分析,表明芸香苷粗提物的纯度较低,纯化后的峰样品纯度98.6%,达到了化学对照品的要求。结论该方法重复性好,分离效果好,可为HSCCC在其他植物黄酮类提取中的应用提供有价值的参考。

Objective To develop a experimental system for the separation of rutoside from Hibiscus mutabilis leaves by high-speed counter-current chromatography （HSCCC）. Methods HSCCC was successfully applied to purify rutoside extracts of Hibiscus leaves. Firstly, crude extract of Hibiscus mutabilis leaves was prepared by water extraction. Then the crude extract was applied to macroporous resin and eluted with 60% ethanol . The above extract was further sperated by HSCCC. The two-phase solvent system used in HSCCC was ethyl acetate ： n-butanol ： water （ 1 ： 0.3 ： 1.5 ）. Results The purity of the obtained rutoside was over 98.6%. Conclusion HSCCC is a feasible method for the ourification of rutoside from the herb.