三价铁对灯盏花素与牛血清白蛋白相互作用的影响

Effect of ferric iron on interaction between breviscapinun and bovine serum albumin

运用荧光光谱、紫外吸收光谱探讨了Fe3+对灯盏花素(BR)与牛血清白蛋白(BSA)相互作用的影响;从Fe3+与BSA的静电作用、配位结合以及Fe3+与BR的配位作用等方面分析了影响BR与BSA相互作用的因素.结果表明,Fe3+不改变BSA与BR的作用机制,但使得二者结合的猝灭常数、结合常数及结合位点数减小.

The effect of ferric iron （Fe3＋ ） on the interaction between breviscapinun （BR） and bovine serum albumin （BSA） was investigated by means of fluorescence spectrometry and ul- traviolet absorption spectrometry. The factors influencing the interaction between BR and BSA were analyzed in relation to the static action and coordination of Fe3＋ and BSA as well as the coordination action between Fe3＋ and BR. Results indicate that Fea＋ does not change the mech- anism for BSA to interact with BR, but it contributes to reduce the quenching constant, bind ing constant and binding sites of BSA with BR.