摘要
试验旨在为深入研究单核巨噬细胞的生物学特性和免疫学功能提供材料。应用牛淋巴细胞分离液从牛外周血中分离牛外周血单个核细胞,用含10%胎牛血清的RPMI1640营养液培养5d,在倒置显微镜下观察细胞形态,采用流式细胞术检测单核巨噬细胞表面特征性标志CD14。结果表明,获得的贴壁细胞具备巨噬细胞的形态学特征及免疫表型。通过形态学观察和流式细胞术鉴定可知,该试验成功获得了牛外周血单核巨噬细胞。
To provide materials for further study on the biological characteristics and immunological functions of monocyte macrophage,bovine peripheral blood mononuclear cells were isolated by using bovine lymphocyte separation medium and incubated in RPMI 1640 medium supplemented with 10% fetal bovine serum for 5 days. The cell morphology was observed by inverted microscope and surface characteristic marker CD14 of monocyte macrophages was detected by using flow cytometry. The results showed that the obtained adherent cells had the morphological characteristics and immunophenotype of macrophage. Through the morphological observation and identification by flow cytometry, bovine peripheral blood monocyte macrophage was successfully obtained.
出处
《畜牧与饲料科学》
2013年第5期21-22,25,共3页
Animal Husbandry and Feed Science
基金
国家自然科学基金项目(30860202)
内蒙古自然科学基金项目(2010BS0407)
关键词
牛
单核巨噬细胞
分离
鉴定
流式细胞术
形态学观察
bovine
monocyte macrophage
isolation
identification
flow cytometry
morphological observation