牛外周血单核巨噬细胞的分离、培养与鉴定

Isolation, Culture and Identification of Bovine Peripheral Blood Monocyte Macrophages

试验旨在为深入研究单核巨噬细胞的生物学特性和免疫学功能提供材料。应用牛淋巴细胞分离液从牛外周血中分离牛外周血单个核细胞,用含10%胎牛血清的RPMI1640营养液培养5d,在倒置显微镜下观察细胞形态,采用流式细胞术检测单核巨噬细胞表面特征性标志CD14。结果表明,获得的贴壁细胞具备巨噬细胞的形态学特征及免疫表型。通过形态学观察和流式细胞术鉴定可知,该试验成功获得了牛外周血单核巨噬细胞。

To provide materials for further study on the biological characteristics and immunological functions of monocyte macrophage,bovine peripheral blood mononuclear cells were isolated by using bovine lymphocyte separation medium and incubated in RPMI 1640 medium supplemented with 10% fetal bovine serum for 5 days. The cell morphology was observed by inverted microscope and surface characteristic marker CD14 of monocyte macrophages was detected by using flow cytometry. The results showed that the obtained adherent cells had the morphological characteristics and immunophenotype of macrophage. Through the morphological observation and identification by flow cytometry, bovine peripheral blood monocyte macrophage was successfully obtained.