鼻咽癌染色体7q32区域微缺失的研究

Detailed allelic loss mapping on 7q32 in nasopharyngeal carcinoma *

目的 进一步明确鼻咽癌染色体 7q32的 D7S5 0 0 - D7S495附近区域高频率等位基因缺失的范围。方法 采用更高密度的微卫星标记位点 ,分析 30个鼻咽癌病例等位基因杂合性缺失的情况。结果30例患者中有 19例 (6 3.3% )存在杂合性缺失 ;D7S5 0 0 - D7S5 0 9- D7S495是高频率缺失集中的区域 ,共同缺失区在 D7S5 0 9附近。结论 D7S5 0

Objective The present research group had found high frequency of loss of heterozygosity (LOH) of D7S500 D7S495 on chromosome band 7q32 in nasopharyngeal carcinoma, this study was conducted to further find the deletion region. Methods Higher density of loci on 7q32 in 30 tumors was studied by using microsatellite analysis. Results The frequency of LOH was 63.3%. The highest frequency of LOH was identified at the loci of D7S500 D7S509 D7S495, among which a common deletion of D7S509 was found.Conclusion A putative tumor suppressor gene may be present around D7S509.