川芎嗪对大鼠视网膜缺血再灌注损伤的保护作用

Effect of Ligustrazine for Protecting Retina Ischemia-reperfusion Injury in Rats

【目的】探讨川芎嗪对大鼠视网膜缺血再灌注损伤的保护作用及其机制。[方法]SD大鼠30只，随机分为对照组、缺血再灌注组、川芎嗪组，每组10只。建立大鼠视网膜缺血再灌注损伤模型，川芎嗪组在麻醉前30min和其后6h分别经腹腔注射川芎嗪30mg／kg各一次。缺血再灌注后12h、24h分别检测视网膜超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量及观察视网膜组织的病理学变化。【结果】与对照组比较，缺血再灌注组SOD活性显著降低，MDA含量显著升高（P〈0．05），视网膜组织出现明显病理损伤；川芎嗪组较缺血再灌注组SOD活性显著升高，MDA含量显著降低（P〈0．05），视网膜组织损伤程度较轻。【结论】川芎嗪对大鼠视网膜缺血再灌注损伤具有保护作用，抑制氧自由基反应可能是其重要机制。

[Objective]To explore the protection and mechanism of ligustrazine on retinal isehemia-reperfusion in- jury in rats. [Methods]Thirty SD rats were randomly divided into control group, ischemia-reperfusion group and ligu- strazine group with 10 in each. Rat retina ischemia-reperfusion injury model was established. Ligustrazine group was intraperitoneally injected with ligustrazine 30mg/kg at 30min before anesthesia and 6h after anesthesia. Superoxide dismutase（SOD） and malonaldehyde （MDA） in retinal at 12h and 24h after ischemic repeffusion were detected. Renal histopathological lesion was observed. [Results]Compared with control group, SOD in ischemia-reperfusion group decreased significantly and MDA increased significantly（ P 〈0.05）. Renal tissues had obvious pathological lesion in ischemia-reperfusion group. Compared with ischemia-reperfusion group, SOD significantly increased and decreased in li gustrazine group（ P 〈0.05）. The pathological lesion in ligustrazine group was lighter than that in ischemia-reperfu- sion group. [Conclusion]Ligustrazine has protective effect on retinal ischernia-reperfusion injury in rats. Its mechanism may be related to the inhibition of oxygen free radical reaction.