医用抗病毒剂脱除葡萄病毒的初步研究

Primary Study on the Virus Elimination of Grapevine by Medical Antiviral Compound

以携带葡萄(Vitis spp.)斑点病毒(GFkV)、沙地葡萄茎痘伴随病毒(GRSPaV)、葡萄病毒A(GVA)、葡萄卷叶伴随病毒-1(GLRaV-1)、葡萄卷叶伴随病毒-2(GLRaV-2)、葡萄卷叶伴随病毒-3(GLRaV-3)和葡萄卷叶伴随病毒-6(GLRaV-6)等病毒的8个葡萄品种试管苗样品为材料,采用不同质量浓度的医用抗病毒剂利巴韦林注射液进行脱毒处理。结果发现,葡萄植株的死亡率较高,设置的4个质量浓度处理中,只有加入25 mg/L利巴韦林注射液处理的试管苗样品获得了再生植株。对分离成活的茎尖进行RT-PCR检测,结果表明,获得的5个葡萄品种的9株再生植株中,只有维多利亚的2株检测带有GRSPaV,其余均不带有所检病毒。

Eight in vitro grapevine （Vitis spp. ） species infected by Grapevine spot virus （GFkV）, Grapevine rupestris stem pitting- associated virus （GRSPaV）, Grapevine virus A （GVA）, Grapevine leaf-roll associated virus- 1 （GLRaV- 1 ）, Grapevine leaf-roll associated virus-2 （GLRaV-2）, Grapevine leaf-roll associated vires-3 （GLRaV-3） or Grapevine leaf-roll asseciated virus-6 （GLRaV-6） were used as original materials. The elimination rates of medical antiviral compound in four different concentrations and ribavirin injection were analyzed. In the course of processing, the mortality rate of grapevine was higher, and nine regeneration plants from five species were obtained only from 25 mg/L ribavirin injection treatment. Viruses in regenerated plants were detected by RT-PCR, and the results showed that only two regeneration plants of Victoria still contained GRSPaV, while the rest were virus-free.