乳果糖口服液含量的HPLC测定法研究

Development of HPLC Method for Lactulose Oral Solution Assay

目的 :建立一种乳果糖口服液含量的反相高效液相色谱测定法。方法 :氨基柱 ,以磷酸盐缓冲液 -乙腈(19∶81)为流动相 ,流速 1 8mL·min-1,检测器 :示差折光检测器。结果 :线性范围 :5 0～ 40 0 μg ,r =0 9999,回收率为 10 0 0 % ,RSD为 1 0 % (n =5 )。结论 :本方法结果准确 ,重现性好 ,为乳果糖口服液提供有效的质量控制方法。

Objective:A HPLC method was described for the determination of lactulose Method:NH 2 column,with a mixture of acetonitrile-phosphate buffer(81∶19) pH 6 as mobile phase The refractive index detector was used Results:The standard curve was linear in the range of 50～400 μg of lactulose( r=0 999 9) The average recovery of lactulose was 100 0%( n=5),RSD =1 0% Conclusions:The established method is rapid,accurate and reliable This method is significant for the quality control of lactulose and its relative substances