N-糖基化抑制对胶质瘤细胞膜流动性影响的研究

Investigation on the influece of N-glycosylation inhibitor on the mobility of glioma cell membrane

目的 为了研究复合糖分子变异对胶质瘤细胞膜流动性的影响。方法 采用共聚焦激光显微镜 ,运用FRAP分析技术 ,观察了N 糖基化抑制剂Tunicamycin作用前后胶质瘤细胞膜流动性的变化。结果 Tunicamycin抑制胶质瘤细胞膜的流动性。结论 胶质瘤细胞膜的流动性与其细胞膜上糖蛋白的N

Objective To study the influence of the alteration of carbohydrate molecules to the cell membrane mobility. Methods Tunicamycin was used as the inhibitor of the Nlinkedglycosylation. A part of glioma cell membrane was bleached by laser, and the fluorescence redistribution was measured by adherent cell analysis and sorting cytometer 570 (ACAS 570). Results The result demonstrated that tunicamycin inhibited the mobility of the glioma cell membrane. Conclusion Inhibition of Nlinkedglycosylation can influence on the mobility of tumor cell membrane.