摘要
为观察微波对体外培养的猪视网膜色素上皮细胞的脂质过氧化损伤作用及锌的生物防护作用 ,体外培养猪视网膜色素上皮细胞 ,微波按强度分为 3组 (10、2 0、30 m W· cm- 2 )辐照 1h。 30 m W· cm- 2 为防护组 ,在培养液中加入不同浓度的硫酸锌。辐照后立即测定 SOD活性、MDA含量 ,检测细胞存活率。结果显示微波辐照后 ,SOD活性下降 ,MDA含量升高 ,细胞存活率明显下降 ,加入锌后 ,可减轻这种损伤。在本实验条件下 ,微波可引起体外培养的猪视网膜色素上皮细胞的脂质过氧化损伤 ,锌可提高细胞的抗氧化能力 ,减轻细胞的损伤。
To observe the lipid peroxide damage in the cultured pig retinal pigment epithelial cell(RPE) induced by microwave and the protective effects by zinc (Zn).RPE cells were divided into 3 groups in terms of microwave intensity(the group of 10, 20 and 30mW·cm -2 )and each group was exposed to microwave for 1 hour.After adding ZnSO 4 into DMEM culture fluid,the group of 30mW·cm -2 was exposed to microwave.The activity of SOD and the content of MDA were measured.The results showed that the activity of SOD decreased significantly compared with the control group( P<0 001 ).The content of MDA increased significantly compared with the control group( P <0 001).Administration of Zn alleviated the increase of MDA and decrease of SOD.In the experiment,2450MHz microwave induces the lipid peroxide damage in RPE cells. Zn can enhance the antioxidation ability of cells and alleviate the damage to some extent.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第3期129-131,共3页
Journal of Hygiene Research
基金
国家自然科学基金!资助项目 ( No.3 970 0 119)
