杂色曲霉素致人胚肺细胞p53及Ki-ras基因突变研究

Mutation of p53 and Ki-ras gene in human fetal lung fibroblast cells in vitro by sterigmatocystin

为探讨中国恶性肿瘤高发区粮食中优势污染霉菌毒素—杂色曲霉素 (Sterigmatocystin,ST)的致癌作用 ,运用银染 PCR- SSCP方法分析了不同浓度的 ST(1μg/ ml和 3μg/ m l)对体外培养的人胚肺细胞恶性转化过程中抑癌基因 p5 3第 5、6、7、8外显子及癌基因 Ki- ras的突变情况。结果显示 ST处理后第 2 2周 ,人胚肺成纤维细胞 p5 3基因的第 8外显子和 Ki- ras癌基因均出现异常泳动带型 ,表明 ST诱发了抑癌基因 p5 3及癌基因 Ki- ras突变 ,进一步证实了 ST对人肺组织的致癌作用。

To explore the carcinogenic effects of sterigmatocystin(ST), one of the predominant contaminating mycotoxins in high risk areas of cancer in China, mutation of tumor suppressor gene p53 and oncogene Ki ras in human fetal lung cells in vitro induced by ST was studied using cell culture and silver staining PCR SSCP methods. The results showed that, within 22 weeks of ST treatment, no abnormalities were found for both p53 and Ki ras gene in electrophoresis. Abnormal electrophoretic migration bands were seen at exon 8 of p53 gene and Ki ras gene in ST treated human lung fibroblast cells 22 weeks after ST treatment. Thus, the results further confirmed the carcinogenic effects of ST on human fetal lung tissue.