贯叶金丝桃的细胞培养

Study on the Callus and Cell Culture of Hypericum perforatum

从贯叶金丝桃叶的嫩茎诱导愈伤组织的过程，探讨了悬浮培养和平板培养的方法，以及细胞悬浮培养时间、培养方法和接种密集度对贯叶金丝桃单细胞平板培养植板率的影响。在所实验的ＭＳ、Ｂ５、Ｗｈｉｔｅ３种培养基中，以ＭＳ培养基较好，其中ＭＳ＋２，４－Ｄ１ｍｇ／Ｌ＋ＮＡＡ １ ｍｇ／ｌ培养基愈伤组织诱导率为９２．８６％。细胞悬浮培养以Ｂ５培养基较好。普通单细胞平板培养法培养贯叶金丝桃单细胞的植板率很低。

Callus were induced from the leaves and stems of Hypericum perforatun L. The cell suspension culture and plate culture were also investigated in this paper.The results showed that the medium of MS which contained 2,4-D Img/L and NAA Img/L was the best one for inducing callus.The plant growth regulator of 2,4-D was assential for callus and suspension culture. Factors such as the time of cell suspension culture, the ways of plating and cell density, which affects the plating efficiency were studied.The results showed that plating at density of 5×103 cells/ml and with monocells seperated from the cells cultured 14 -21 days,high plating efficiency could be obtained either by conditional plate culture or by nursing plate culture.