TLR-2和TLR-5在弥漫大B细胞淋巴瘤组织中的表达及临床意义

Expression and clinical significance of TLR-2 and TLR-5 in diffuse large B-cell lymphoma

目的探讨Toll样受体-2(TLR-2)和Toll样受体-5(TLR-5)在弥漫大B细胞淋巴瘤(DLBCL)组织中的表达及其临床意义。方法采用免疫组化法检测60例DLBCL组织标本中TLR-2和TLR-5的表达,以21例反应性淋巴结增生组织为对照组,并分析TLR-2和TLR-5表达与DLBCL临床病理特征的关系。结果 TLR-2在DLBCL组织中的阳性表达率为78.3%,在对照组中为28.5%,差异有统计学意义(P<0.05);TLR-2表达与年龄、性别和ECOG评分无关,与临床分期、乳酸脱氢酶水平、B症状、IPI、免疫亚型以及结外受累有关。TLR-5在DLBCL中的阳性表达率为26.6%,在对照组中为23.8%(P>0.05);TLR-5表达与各临床病理特征无关。结论 TLR-5与DLBCL临床特征无关;TLR-2可能参与了DLBCL的发生和发展,有望作为判断其预后的生物学指标。

Objective To investigate the expression of Toll-like receptor-2 （TLR-2） and Toll-like receptor-5 （TLR-5） in dif- fuse large B cell lymphoma（DLBCL） , as well as the relationship of their expression with clinical features. Methods Immunobisto- chemistry stain was used to determine the expression of TLR-2 and TLR-5 in 60 DLBCL tissues. Twenty-one lymph node reactive hy- perplasia tissues were taken as control. The relationship between TLR-2, TLR-5 expression and clinical features was analyzed. Results Positive rates of TLR-2 in DLBCL tissues and control group were 78.3% and 28.5% （P 〈 0. 05 ）. The expression of TLR-2 was re- lated with clinical stage, LDH, IPI, subtypes, out of lymph node invasion and systemic symptoms, but not related to age, gender and ECOG score. Positive rates of TLR-5 in DLBCL tissues and control group were 26. 6% and 23.8% （P 〉0. 05）, and there was no rela- tionship between it and clinical features. Conclusion TLR-5 has no relationship with clinical features of DLBCL;TLR-2 may be relat- ed to the occurence and prognosis of DLBCL, could be regarded as an biomarker of prognosis of this disease.