散发性乳腺癌中MTA1表达与ERα甲基化关系的研究

Relation between estrogen receptor-alpha promoter methylation and MTA1 expression in sporadic breast cancer

目的:通过甲基化特异性PCR和免疫组化研究女性散发性乳腺癌中ERα基因启动子区甲基化和MTA1蛋白表达的相关性。方法:甲基化PCR研究102例散发性乳腺癌ERα启动子甲基化情况,免疫组化研究其MTA1蛋白表达情况。结果:乳腺癌组织中ERα启动子甲基化率为37.3%,乳腺癌组织中MTA1表达率为29.4%,高于其在癌旁正常乳腺组织中的表达(P<0.05),MTA1表达和ERα启动子甲基化均与乳腺肿瘤大小、TNM分期及淋巴结转移相关(P<0.05),且乳腺癌中ERα启动子甲基化与MTA1阳性表达呈正相关(P<0.05)。结论:女性散发性乳腺癌中ERα基因启动子甲基化与MTA1表达升高密切相关,其在乳腺癌发展过程中可能起重要作用。

Objective：To study the relation between the promoter methylation of estrogen receptor-alpha and MTA1 expression in sporadic breast cancer by methylation-specific polymerase chain reaction and immunohistochemistry.Methods：The methylation of ERα promoter was investigated by methylation-specific polymerase chain reaction and MTA1 expression was analyzed by immunohistochemistry in 102 sporadic breast cancer.Results： ERα methylation was observed in 37.3% breast cancer tissues and MTA1 expression in breast cancer（30/102,29.4%） was higher than that in non-neoplastic breast tissues（6/102,5.9%）（P0.05）.Both of them were significantly associated with lymph node metastasis and tumor size and TNM stage in breast cancer（P0.05）.ERα methylation had significant correlation with the MTA1 expression（P0.05）.Conclusion：The ERα methylation status had tight relation with MTA1 expression in female sporadic breast cancer,and they may play important roles in the carcinogenesis of breast cancer.