炎症标志物白细胞介素-13在多囊卵巢综合征患者外周血的表达水平及临床意义

Clinical significances and expressions of inflammatory marker interleukin-13 in peripheral blood of patients with polycystic ovary syndrome

目的:观察白细胞介素-13(interleukin-13,IL-13)在多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者外周血中的表达水平,并分析其与患者体质指数(body mass index,BMI)、生殖相关激素、血糖及血脂的相关性。方法:临床对照试验。研究对象分为3组:PCOS肥胖组(40例)、PCOS非肥胖组(35例)和对照组(39例)。测量各组研究对象的体质量、身高、腰围及臀围,并计算BMI及腰臀比(waist/hip ratio,WHR)。分别用酶联免疫吸附试验测定血清IL-13水平;应用化学发光法检测空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting insulin,Ins)、胆固醇(cholesterol,TC)、甘油三酯(triglycerides,TG)、高密度脂蛋白(high-density lipoprotein,HDL)、低密度脂蛋白(low-density lipoprotein,LDL)水平;放射免疫测定:卵泡刺激素(follicle-stimu-lating hormone,FSH)、黄体生成素(luteum erythropoietin,LH)、雌二醇(estradiol,E2)、孕酮(progesterone,P)、催乳素(prolactin,PRL)、睾酮(testosterone,T)。结果:PCOS肥胖组外周血IL-13的表达高于非肥胖组(P=0.000),且二者均高于对照组(P=0.000;P=0.019)。Ins、LDL、HDL、TG、TC、IR、E2、FSH、P等指标的血清水平无统计学差异;而WHR、BMI、FBG、LH、T等指标血清值在PCOS肥胖组、非肥胖组均高于对照组。简单相关回归分析显示IL-13与BMI(R=0.527,P=0.000)、FBG(R=0.449,P=0.000)及T(R=0.452,P=0.000)、WHR(R=0.272,P=0.021)及LH(R=0.247,P=0.037)有相关性,多元线性回归方程表示为IL-13=90.814+1.255BMI+0.755WHR+1.696FBG+0.146LH+0.738T。结论:炎症标志物IL-13在PCOS患者外周血中表达明显升高,并与BMI、WHR、FBG、LH及T等指标存在线性依存关系。

Objective：To observe the expressions of interleukin-13 （IL-13） in peripheral blood of patients with polycystic ovary syn- drome （PCOS） and to analyze their relationship with the body mass index （BMI）, reproductive relevant hormone, fasting blood glucose （FBG） and blood fat. Methods：Clinical control trial was carried out. All patients were assigned to three groups：PCOS with obesity （40 cases）, PCOS with non-obesity （35 cases） and controls （39 cases）. Body weight, body height,waist circumference and hip cir- cumference were measured and BMI and waist/hip ratio（WHR） were calculated. Enzyme-linked immuno sorbent assay（ELISA） kit for IL-13 was used to test the expression in peripheral blood. Chemiluminescence method was applied to detect FBG,fasting insulin （Ins） ,cholesterol（TC）,triglycerides（TG）,high-density lipoprotein（HDL） and low-density lipoprotein（LDL） level for the patients in three groups. Reproductive hormones including follicle-stimulating hormone （FSH）, corpus luteum erythropoietin （LH）, estradiol （E2）, progesterone（P）,prolactin（PRL） and testosterone（T） were assayed by radiation immunoassays（RI）. Results：Expression of IL-13 in peripheral blood was higher in PCOS with obesity than in PCOS with non-obesity（P=0.000） and that was higher in PCOS with obesi- ty and PC OS with non-obesity than in controls（P=0.0OO;P=0.019）. Serum levels of Ins, LDL, HDL,TG,TC, IR, E2, FSH and P were not statistically different. Serum levels of WHR, BMI, FBG, LH and T were higher in PCOS with obesity and PCOS non-obesity than in controls. Simple correlated regression analysis showed that IL-13 was correlated with BMI（R=0.527,P=0.000）,FBG（R=0.449,P= 0.000）,WHR （R=0.272,P=0.021）,LH（R=0.247,P=0.037） and T（R=0.452,P=0.000） and multiple linear regression formula was IL- 13 =90.814＋ 1.255BMI＋0.755WHR＋ 1.696FBG＋0.146LH ＋0.738T. Conclusions ： Expression of inflammatory marker IL- 13 is in- creased in the peripheral blood of PCOS patients and has positive correlation with BMI,WHR,FBG,LH and T indexes.