兔急性细菌性心内膜炎的制作方法

A method for the establishment of experimental rabbit endocarditis

目的 :建立一种简单易行而且成功率高的兔细菌性心内膜炎模型。方法 :自麻醉新西兰兔颈总动脉插一导管至左心室 ,2 4h后随机分为对照组和感染组。感染组自耳缘静脉注射 1 0 9cfu甲型溶血链球菌 ,对照组注射 1ml的生理盐水。 7d后抽血进行血液培养 ,处死后观察主动脉瓣赘生物、左心室腔内附壁赘生物及心脏组织学改变。结果 :感染组每只存活到最后的动物在主动脉瓣和左心室底部均有大小不等的赘生物生长 ;血液及赘生物培养液中均有细菌 ;光镜下观察到赘生物外 ,心内膜、心肌组织均见局灶变性、坏死、出血、纤维化和炎性细胞浸润 ,心肌间质水肿 ,并有炎性细胞浸润。而对照组主动脉瓣和左心室底部均无赘生物形成 ;光镜下见心尖部心内膜有损伤、脱落 ;血液培养结果阴性。结论 :用这种方法制作兔实验性细菌性心内膜炎成功率高 ,简单易行。

OBJECTIVE:To establish the rabbit model of experimental endocarditis caused by α hemolytic streptococcus.METHODS:Rabbits were anesthelized we inserted a sterile polythelene cather across the aoritic valve from the right caroid artery into the left ventricle.24h after the operation,the animals were divided into two group:control group and infection group.Each animal of infection group was injected with 10 9 CFU α hemolytic streptococcus through peripheral ear vein.7d after injection,all animals were sacrified,the vegetation at cardial valve and endocardial of the left ventricle were removed,weighted,and the number of organisms in blood and vegetation was counted.RESULTS:Each infection animal had cardial valve vegetation and endocardial vegetation.Infected organisms were found in blood and vegetation.Vegeatations were found at aortic and buttom of left vetricle,and local degeneration,necrosis,fibrosis,inflammatory cell infiltration and interstitial edema in the endocardium and myocardium.The control animals had no vegetation,but had inflammatory injury in the endocardium.CONCLUSION:This method is simple and reliable for making rabbit endocarditis model.