银杏叶醇提取物对异烟肼和利福平肝毒性保护作用的实验研究

Protective effect of ethanol extracts of ginkgo biloba leaves on mice hepatic toxic injury induced by isoniazid and rifampicin

目的 :观察银杏叶醇提取物对异烟肼和利福平肝毒性的保护作用及其机制探讨。方法 :分别测定肝损害组和银杏叶醇提取物大、小剂量组小鼠的血清谷丙转胺酶 (SGPT)、肝指数、肝匀浆丙二醛 (MDA)含量、肝微粒体P4 50和线粒体Ca2 + ATP酶活性 ,以及肝病理检查 ,并与对照组比较。结果 :银杏叶醇提取物大、小剂量均可对抗异烟肼和利福平引起的MDA、SGPT、肝微粒体P4 50 的增高 (P <0 .0 5) ,以及对抗其引起的形态学改变 ;银杏叶醇提取物大剂量对抗其线粒体Ca2 + ATP酶活性的降低。结论 :银杏叶醇提取物可对抗异烟肼和利福平所致肝毒性。

OBJECTIVE:To observe the protective effect of ethanol extracts from ginkgo biloba leaves on mice hepatic toxic injury induced by isoniazid and rifampicin.METHODS:The level of SGPT,liver index,content of malondialdehyde(MDA) of liver homogenate,activity of hepatic microsome enzyme and mitochondrial membrane Ca 2+ ATP enzyme,and degree of liver cell injury were measured.RESULTS:The ethanol extracts from ginkgo biloba leaves decreased the level of SGPT,liver index,content of MDA of liver homogenate,activity of hepatic microsome enzyme(P<0.05),and the degree of liver injury.On the other hand,ethanolic extracts from ginkgo biloba leaves increased the activity of mitochondrial membrane Ca 2+ ATP enzyme.CONCLUSION:The ethanol extracts from ginkgo biloba leaves had the protective effect on mice hepatic toxic injury induced by isoniazid and rifampicin.