TS和BRCA1在胃癌组织中的表达及其临床意义

Clinical significance of TS and BRCA1 protein overexpression in gastric cancer

目的:评价胸苷酸合成酶(thymidylate synthase,TS)和乳腺癌易感基因1(breast cancer susceptibility gene-1,BRCA1)过表达与胃癌患者临床病理参数间的相关性及其临床意义.方法:收集2011-01/2012-01在中国人民解放军南京军区福州总医院手术切除的临床资料完整的246例胃腺癌标本,采用Elivision plus免疫组织化学染色方法检测胃癌组织中TS和BRCA1的表达.使用SPSS16.0软件进行2检验分析.结果:TS和BRCA1在胃癌组织中阳性率分别为39.02%(96/246)、55.69%(137/246);TS蛋白表达与患者性别、年龄、肿瘤部位、TNM分期、分化程度、浸润深度、淋巴结转移、远处转移均无相关性(P>0.05);BRCA1蛋白表达与患者性别、年龄、肿瘤部位、TNM分期、分化程度远处转移无相关性(P>0.05),而与浸润深度(P<0.01)和TNM分期(P<0.05)相关.胃癌组织中TS和BRCA1共同表达阳性率为26.02%(64/246),联合分析显示TS和BRCA1表达呈负相关(P<0.01,Pearson列联系数C=0.2472).结论:胃癌组织中存在TS和BRCA1过表达,BRCA1蛋白表达与TNM分期和浸润深度相关,提示BRCA1表达和肿瘤侵袭性具有相关性,可作为评估胃癌生物学行为以及预后的参考指标.BRCA1和TS的表达检测有助于临床化疗药物的选择.

AIM： To investigate the correlation between thymidylate synthase （TS） and breast cancer sus- ceptibility gene-1 （BRCA1） expression and clini- copathological characteristics of gastric cancer. METHODS： Two hundred and forty-six surgical specimens of gastric cancer collected from patients with complete clinical data who were treated at Fuzhou General Hospital of Nanjing Military Command between January 2011 and January 2012 were used in this study. The protein expression of TS and BRCA1 in these specimens was examined by immunohistochemistry. The correlation between TS and BRCA1 protein expression and clinicopathological characteris- tics of gastric cancer was analyzed. RESULTS： The rates of TS and BRCA1 overex- pression in gastric cancer were 39.02% （96/246） and 55.69% （137/246）, respectively. There was no relationship between TS overexpression and sex, age, tumor site, histotype, differentiation, distant metastasis, depth of invasion, TNM stage and lymph node metastasis （all P 〉 0.05）. BRCA1 protein overexpression was associated with depth of invasion （P 〈 0.01） and TNM stage （P 〈 0.05）, but not with sex, age, tumor site, differentiation, lymph node metastasis or distant metastasis （all P 〉 0.05）. The co-expression rate of TS and BRCA1 in gastric cancer was 26.02% （64/246）. The overexpression of TS was negatively correlated with that of BRCA1 （P 〈 0.01, r = 0.2472）. CONCLUSION： There exists TS and BRCA1 overexpression in gastric cancer. The overexpression of BRCAlwas associated with TNM stage and depth of invasion, which implies that BRCA1 overexpression may be related to invasion of gastric cancer. Detection of BRCA1 protein overexpression may be used to assess the malignant biological behavior and prognosis of gastric cancer and help choose chemotherapy drugs.