Klotho基因多态性与衰老的相关性研究

Relationship study on the Klotho gene polymorphism and aging

目的研究Klotho基因多态性与中国汉族人群衰老的关系。方法在482例(<60岁236例,60岁246例)沈阳地区健康汉族人群,采用Snapshot(微测序)的方法检测Klotho基因标签SNP(TagSNP)-rs571118位点和功能SNP位点rs9536314(F352V),rs9527025(C370S)的基因型,观察Klotho基因多态性与衰老的关系。结果证实在中国汉族人群F352V(rs9536314)为G纯合子,C370S(rs9527025)为T纯合子;rs571118位点存在AA,GG,AG基因型。老年人群基因型分布为(AA35(14.22),AG132(53.65),GG79(32.11)),年轻人群基因型分布为(AA51(21.61),AG128(54.23),GG57(24.15))。Klotho基因rs571118位点G等位基因频率在老年人群高于年轻人群。结论 Klotho基因rs571118位点多态性与衰老存在明显相关。

Objective To study the relationship between the Klotho gene polymorphism and Chinese Han population aging. Methods The genotypes of Klotho TagSNP rs571118 and rs9536314 （F352V）, rs9527025 （ C370S ） of 482 heathy Han peoples in Shenyang were detected by using Snapshot（mini-sequencing） method,and the possible relationship between the Klotho gene polymorphism and aging was observed. Results It were confirmed that the F352V （rs9536314）was G homozygous,the C370S （rs9527025）was T homozygous and the genotypes of AA,GG and AG were exited in rs571118 in Chinese Han population.The genotype distribution in aging population were AA35（14.22）,AG132（53.65） and GG79（32.1 1）,while the genotype distribution in young population were AA51（21.61）,AG128（S4.23） and GG57（24.15）.The G allele frequency in Klotho gene rs571118 in aging population was higher than that in young population. Conclusion Klotho gene rs571118 polymorphism has significant correlation with aging.