摘要
目的 :测定痤疮霜中甲硝唑和氯霉素的含量。方法 :高效液相色谱法 ,以地塞米松为内标 ,Nova PakC18柱 ,甲醇 水为流动相 ;测定波长 2 60nm。结果 :一次进样可完成 2种化学性质不同组份的含量分析。甲硝唑 9.94~1 0 9.3 4 μg/ml,氯霉素 4 .92~ 54.1 2 μg/ml范围内 ,峰面积与其浓度呈良好的线性关系 ;平均回收率依次为 97.3 1 % ,RSD =0 .94 % ;98.1 2 % ,RSD =0 .78% (n =5)。结论 :方法简便 ,结果满意 ,适于医院制剂分析。
OBJECTIVE:To separate and determine the contents of metronidazole and chlormycetin in Zhuochuangshuang.METHODS:A HPLC method was used with a column of Nova Pak C 18 .Dexamethasone was used as internal standard.The mobile phase consist of methanol and water.The detecting wavelength was 260nm.RESULTS:The separation and determination of two constituents were accomplished by only one sample inject.The calibration curves were linear in the range of 9.94~109.34μg/ml for metronidazole,and 4.92~54.12μg/ml for chloromycetin.The average recoveries of metronidazole,chloromycetin were 97.31%(RSD=0.94%),98.12%(RSD=0.78%,n=5) respectively.CONCLUSION:This method was simple and quick for the simultaneous determination of the contents in Zhuochuangshuang.
出处
《中国现代应用药学》
CAS
CSCD
2000年第3期249-251,共3页
Chinese Journal of Modern Applied Pharmacy