人工合成冬虫夏草对血糖和胰岛素分泌影响的实验研究

Experiment Study on Effects of Artificial Cordyceps Sinensis (Q80) on Lowering Blood Glucose and Excreting Function of Endocrine Cell in Islets

为探讨人工合成冬虫夏草 (Q80 )对环孢素引起的糖尿病小鼠的血糖和胰岛素分泌的影响 ,用12 5I 胰岛素放射免疫法和葡萄糖氧化法 ,检测了口服和腹腔注射Q80小鼠的胰岛素和血糖浓度。发现 :①注射和口服不同剂量的环孢素均可使小鼠血糖浓度升高 ,胰岛素浓度下降 ,且与正常值比较有显著性差异 (P <0 .0 1 ) ,停药后可恢复正常。②注射或口服环孢素 +Q80后血糖浓度均达到正常 ,而胰岛素浓度均未达正常。③单独使用Q80不能引起血糖浓度的改变 ,口服Q80对胰岛素浓度无影响 ,注射则引起其浓度变化。④单独使用环孢素组与口服环孢素 +Q80组比较 ,后者胰岛素浓度有所提高 ,停药后 2周可恢复正常 ,与注射环孢素 +Q80组比较 ,胰岛素浓度于用药后 2周有所提高 ,停药后反而下降。结果提示 :①注射或口服环孢素均可导致小鼠血糖浓度增高及胰岛素浓度下降。②中药Q80对环孢素引起的血糖升高具有明显的降血糖的作用 ,而单独用药则不影响血糖浓度。③口服和注射Q80对环孢素引起的胰岛素分泌下降有一定的抑制作用 ,但影响不大。④环孢素对血糖的影响是暂时的、可逆的 ,一旦停药 ,血糖即可恢复正常 ,即使加大环孢素的剂量 ,停药后血糖亦可恢复正常。

This study is to determine effect of Q80 on lowering blood glucose and excreting function of endocine cell in islets of diabetic mice by cyclosporine. The diabetic mice resulted from cyclosporine levels were determined by 125 I insulin radioimmunoassay and glucoseoxidation assay. ① Different dose of cyclosporine could heighten the blood glucose and lower the insulin of mice . But the blood glucose and insulin levels recovered to normal levels when withdrawal. And there were significant differences between the blood glucose increase and normal or the insulin reduce and normal. ② The blood glucose of the mice got to normal and insulin could not get to normal after cyclosporine plus Q80 treated by intra abdomial or intro oral way. ③ Using Q80 alone could not change the blood glucose, but Q80 injection could resulted in difference of insulin level. ④ The cyclosporine alone group was compared with Q80 combing with cyclosporine infra oral group in which the insulin level increased and recoved to normal level after two weeks. In the Q80 combining with cyclosporine intro abdomen group, the insulin level increased two weeks after treatment and reduced to lower level than normal after cyclosporine withdrawal. The result showed that: ① Cyclosporine could increase blood glucose level and reduce insulin level by both intra abdomial and intra oral way . ② Herb Q80 had significant effect of lowering blood glucose resulted from cyclosporine. ③ Q80 taken by oral or injection had some inhibit function on the lowering of insulin secreting led by cyclosporine, but no significant difference. ④ The effect of cyclosporine was temporary, the blood glucose would recover to normal once withdrawal, even if the dose of cyclosporine was added to large enough.